1、BRITISH STANDARDBS EN ISO 6888-3:2003IncorporatingCorrigenda Nos. 1,2 and 3Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positivestaphylococci(Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbersThe European
2、Standard EN ISO 6888-3:2003 has the status of a British StandardICS 07.100.30g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58BS EN ISO 6888-3:2003Th
3、is British Standard was published under the authorityof the Standards Policy and Strategy Committee on28 March 2003 BSI 2006ISBN 0 580 41504 XNational forewordThis British Standard is the official English language version of EN ISO 6888-3:2003. It is identical with ISO 6888-3:2003. This standard sup
4、ersedes BS 4285-3:10.2:1989 and BS 4285-3.10.3:1989, which are withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/3, Microbiology, which has the responsibility to:A list of organizations represented on this committee can be obtained on request to its secretary.
5、Cross-referencesThe British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogueunder the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Electronic Catalogue or of
6、British Standards Online.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.Compliance with a British Standard does not of itself confer immunity from legal obligations.Summary of pagesThis document comprises a f
7、ront cover, an inside front cover, the EN ISO title page, the EN ISO foreword page, the ISO title page, pages ii to v, a blank page, pages 1 to 11, the Annex ZA page, an inside back cover and a back cover.The BSI copyright date displayed in this document indicates when the document was last issued.
8、aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK.Amendments issued
9、since publicationAmd. No. Date Comments14536Corrigendum No. 112 June 2003 Correction to EN ISO foreword page and addition of Annex ZA page16083Corrigendum No. 213 February 2006 Correction to EN ISO foreword page16270Corrigendum No. 3July 2006 Addition of supersession details to national forewordEURO
10、PEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 6888-3March 2003ICS 07.100.30English versionMicrobiology of food and animal feeding stuffs - Horizontalmethod for the enumeration of coagulase-positive staphylococci(Staphylococcus aureus and other species) - Part 3: Detectionand MPN technique for lo
11、w numbers (ISO 6888-3:2003)Microbiologie des aliments - Mthode horizontale pour lednombrement des staphylocoques coagulase positive(Staphylococcus aureus et autres espces) - Partie 3:Recherche et mthode NPP pour les faibles nombres (ISO6888-3:2003)Mikrobiologie von Lebensmitteln und Futtermitteln -H
12、orizontales Verfahren fr die Zhlung von koagulase-positiven Staphylokokken (Staphylococcus aureus undandere Species) - Teil 3: Nachweis und MPN-Verfahren frniedrige Keimzahlen (ISO 6888-3:2003)This European Standard was approved by CEN on 28 February 2003.CEN members are bound to comply with the CEN
13、/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any
14、CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN membe
15、rs are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovak Republic, Spain, Sweden, Switzerland andUnited Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATION
16、COMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2003 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 6888-3:2003 ECORRECTED 2003-05-14ForewordThis document (EN IS
17、O 6888-3:2003) has been prepared by Technical Committee ISO/TC 34“Agricultural food products“ in collaboration with Technical Committee CEN/TC 275 “Food analysis- Horizontal methods“, the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, eit
18、her by publication of anidentical text or by endorsement, at the latest by September 2003, and conflicting nationalstandards shall be withdrawn at the latest by September 2003.According to the CEN/CENELEC Internal Regulations, the national standards organizations of thefollowing countries are bound
19、to implement this European Standard: Austria, Belgium, CzechRepublic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and theUnited Kingdom.Endorsement noticeNOTE Normative references t
20、o International Standards are listed in Annex ZA (normative).EN ISO 68883:2003The text of ISO 6888-3:2003/Cor.1:2003 has been approved by CEN as EN ISO 6888-3:2003/AC:20without any modifications.05Reference numberISO 6888-3:2003(E)INTERNATIONAL STANDARDISO6888-3First edition2003-03-15Microbiology of
21、 food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureusand other species) Part 3: Detection and MPN technique for low numbers Microbiologie des aliments Mthode horizontale pour le dnombrement des staphylocoques coagulase positi
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27、 groiiEN ISO 68883:2003iiiContents PageForeword ivIntroduction v1 Scope 12 Normative references . 13 Terms and definitions. 24 Principle . 24.1 Detection method 24.2 Enumeration method 25 Diluents and culture media 36 Apparatus. 67 Sampling 68 Preparation of test sample. 69 Procedure. 69.1 Detection
28、 method 69.2 Enumeration method 79.3 Selection of plates and interpretation. 810 Expression of results 910.1 Detection method 910.2 Enumeration method 911 Precision 1012 Test report 10Bibliography . 11EN ISO 68883:2003ivForeword ISO (the International Organization for Standardization) is a worldwide
29、 federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that
30、committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in acc
31、ordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requ
32、ires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 6888-3 was prepared by Techni
33、cal Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology.ISO 6888 consists of the following parts, under the general title Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureusand other species):
34、 Part 1: Technique using Baird-Parker agar medium Part 2: Technique using rabbit plasma fibrinogen agar medium Part 3: Detection and MPN technique for low numbers EN ISO 68883:2003vIntroduction Because of the large variety of food and feed products, this horizontal method may not be appropriate in e
35、very detail for certain products. In this case, different methods, which are specific to these products, may be used if absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this horizontal method as far as possible. When this part of ISO 6888 is n
36、ext reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has been followed and the reasons for deviations from this method in the case of particular products. The harmonization of test methods cannot be immediate and, for certain grou
37、ps of products, International Standards and/or national standards may already exist that do not comply with this horizontal method. It is hoped that when such standards are reviewed they will be changed to comply with this part of ISO 6888 so that eventually the only remaining departures from this h
38、orizontal method will be those necessary for well-established technical reasons. EN ISO 68883:2003INTENRATIONAL TSANDADR IS-8886 O3:(3002E)1Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other specie
39、s) Part 3: Detection and MPN technique for low numbers 1 Scope This part of ISO 6888 specifies a horizontal method for the enumeration and detection of coagulase-positive staphylococci, using the most probable number (MPN) technique. It is applicable to products intended for human consumption and th
40、e feeding of animals, and environmental samples in the area of food production and food handling. This method is recommended for products where staphylococci are expected to be stressed and in low numbers as, for example, in dried products. Coagulase-positive staphylococci will primarily be Staphylo
41、coccus aureus but Staphylococcus intermedius and some strains of Staphylococcus hyicus also produce coagulase. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated reference
42、s, the latest edition of the referenced document (including any amendments) applies. ISO 6887 (all parts), Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examinationISO 8261, Milk and milk products General guid
43、ance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examinationISO 6888-1:1999, Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part
44、1: Technique using Baird-Parker agar mediumISO 6888-2:1999, Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 2: Technique using rabbit plasma fibrinogen agar mediumISO 7218, Microbi
45、ology of food and animal feeding stuffs General rules for microbiological examinationsISO/TS 11133-1, Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the l
46、aboratoryEN ISO 68883:20032ISO/TS 11133-2:1), Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing culture media3 Terms and definitions For the purposes of this document, the following terms and d
47、efinitions apply. 3.1coagulase-positive staphylococci bacteria which form typical and/or atypical colonies on the surface of a selective culture medium and which show a positive coagulase reaction or a specific rabbit plasma reaction on rabbit plasma fibrinogen agar NOTE For the purpose of this part
48、 of ISO 6888, the confirmation of coagulase-positive staphylococci is based on a strongly positive coagulase reaction, but it is recognized that some strains of coagulase-positive staphylococci give weakly positive coagulase reactions. These latter strains can be confused with other bacteria but the
49、y can be distinguished from such other bacteria by the use of additional tests such as the production of thermonuclease (for details, see IDF 83). 3.2enumeration of the coagulase-positive staphylococci determination of the number of coagulase-positive staphylococci found per millilitre or per gram of sample when the test is carried out according to the method specified in this part of ISO 6888 4 Principle 4.1 Detection method 4.1.1 A selective culture medium is inoculated with a specified
