1、 , ( )INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC) 10444.11 2013(ISO 15214:1998) (ISO 15214:1998, MOD) 2014 10444.11 2013II, - 1.0 92 . - 1.2 2009 . -, . , , , 1 - - ( )2 ( 335)3 , ( 58 28 2013 .) : ( 3166) 004 97 ( 3166) 004 97 AM BY MD - RU TJ UZ 4 ISO 15214:1998 Microb
2、iology of food and animal f ding ff ori on al m od for n m ra ion of m o ilic lac ic acid bac ria olony-co n c ni a 30 ( . . 30 0). ISO T 34 S 9 Microbiology ISO T 34 ood rod c (ISO). ( n). , , . (MOD)5 22 2013 . 1744- 10444.11-2013 (ISO 15214:1998) - 1 2015 .6 10444.11-89 10444.11 2013III - , - . (
3、 ) - . , , 2014 -, 10444.11 2013IV1 10444.11 2013 2015 01 011 - -: - (); - . , 1 - 150 1 3 15 -. , - 1 150 1 3 15 - () . , - 1 1500 1 3 150 - . , -.2 : 6709 72 . ISO 7218 2011 . - 10444.1 84 . , , - , 25336 82 . , 26669 85 . 26670 91 . 29228 91 ( 835-2 81) . . 2. 30425 97 . Microbiology of food and
4、animal feeding stuffs.Methods for detection and enumeration of mesophilic lactic acid bacteria(ISO 15214:1998) 10444.11 2013 2 - - - , 1 , . (), (-) . , , , , .3 :3.1 : Lactobacillus, Leuconostoc, Pedi-ococcus, Streptococcus. 3.2 : , - - 30 37 , , .4 - () , - , , , - .5 , , 5.1 1 4 26669 , .5.2 , 5.
5、2.1 MRS5.2.1.1 , 10,0 , 10,0 , 4,0 (NH4)3C6H5O7, 2,0 (3Na), 5,0 (MgSO4 7H2O), 0,2 (MnSO4 4H2O), 0,05 (K2HPO4), 2,0 (6126), 20,0 (Tween 80), 1,08 12 18,0 ), 3 1000 ) ;) 6709.5.2.2 . - - (. 6.5) , (5,7 0,1) . 25 0. 10444.11 2013 3 ( . 6.1) (121 1) 15 . , - 47 ( . 6.4) , -. , , - 47 ( . 6.4). ( , ), MR
6、S. 1,4 - 10 3 1 /3. . 1000 3 MRS, 47 . (5,7 0,1) . 25 .5.3 5.3.1 , 5,0 , 10,0 , 10,0 , 10,0 , 5,0 12 18,0) , 3 1000) .5.3.2 . - - ( . 6.5) , (7,3 0,1) . 25 . ( . 6.1) (117 1) 15 . , .5.4 5.4.1 , 5,0 , 10,0 , 10,0 , 10,0 , 3 ( 5.4.3) 10,0 , 3 10005.4.2 . - - ( . 6.5) , (7,3 0,1) . 25 . ( . 6.1) (117
7、1) 15 .5.4.3 10444.11 2013 45.4.3.1 , 0,1, 3 100,05.4.3.2 , 100 3, . 3 .5.5 5.5.1 , 10,0 , 10,0, 5,0, 5,0 , 3,0 , 0,5 , 3 ( 5.4.3) 20,0 12 18,0), 3 1000) .5.5.2 . - - (. 6.5) , (7,0 0,2) . 25 . (. 6.1) (121 1) 20 . , .5.6 5.6.1 , 5,0 , 3 25,0 80, 3 0,5MnSO44H2O, 0,1, 0,5, 15,0 18,0), 3 1000,0) .5.6.
8、2 . - (. 6.5) , (6,9 0,1) . 25 . 5 7 3 (. 6.1) (121 1) 15 . . 10444.11 2013 55.7 - = 9,65.7.1 - 10444.1.5.7.2 - , (9,6 0,1) . 25 . 5-7 3 (. 6.1) (121 1) 20 .5.8 - 6,5 % NaCl5.8.1 - 10444.1, 3 100,0NaCl, 60,05.8.2 - . - 5 7 3 (. 6.1) (121 1) 20 .5.9 5.9.1 , 10,0, 3 100,05.9.2 100 3, , , 45 50 , - .5.10 5.10.1 , 1,0 99,8 %, 3 20,0 96 %, 3 50,0, 3 30,05.10.2 , - , . 1 .5.11 5.11.1 , 1,0 96 %, 3 10,0 50 3, 3 100,0 10444.11 2013 65.11.2 . 10 3 - 90 3 .5.12 , . -, . , .6 , . . ISO 7218, 10444.1: 6.1 ( ) ().6.2
