1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) EN 15851 2013 1 (EN 15851:2010, IDT) N 158512013 II , 1.0 92 . 1.22009 . , . , , , 1 - ( ) - ( ) , 4 2 ( 335) 3 , ( 28 2013 . 58-) : ( 3166) 00497 ( 3166) 00497 AM BY KG MD - RU UZ 4 N 15851:2010 Foodstuffs. Determinatio
2、n of aflatoxin B1in cereal based foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection ( . 1 . ). (de) , , . (IDT) 5 17 2013 . 1170- EN 158512013 1 2014 . 6 EN 158512013 III , . () . , , 2014 , N 15851-2013 1 1 Foodstuffs. Determination of af
3、latoxin B1in cereal based foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection 20140701 1 1 () . , 1 0,07 0,18 /. 9 . 2 . . , . EN ISO 3696:1995 Water for analytical laboratory use Specification and test methods (ISO 3696:1987) ( . ) 3 . , -
4、 , , 1, 1. 1 - . , - . 4 4.1 N ISO 3696:1995. . , , , . . (IARC) 1. 4.2 . 4.3 . N 158512013 2 4.4 (Na2HPO4) (Na2HPO4 12H2O). 4.5 . 4.6 . 4.7 . 4.8 . 4.9 . 4.10 , w (HCl) = 37 %. 4.11 , (HCl) = 0,1 /3 8,28 3 4.10 1 3. 4.12 , c (NaOH) = 0,1 /3 4 4.9 1 3. 4.13 - c(NaCl) = 120 /3, c(Cl) = 2,7 /3, c (Na2
5、HPO4+ H2PO4) = 10 /3, = 7,4 8,0 4.8, 1,2 2,9 (Na2HPO412H2O) 4.4, 0,2 4.7 0,2 4.6 900 3. 7,4 . pH 4.11, 4.12, 1 3. . 4.14 - (CAS 39416-48-3). 4.15 . , , , . , , . 4.16 . 4.17 . 4.18 . 4.19 8 4.17 . 4.20 , (HNO3) = 4 /3. 4.21 - 4.15 4.16. , , 4.2. 4.22 , 4.15 4.16. 1 3 120 4.5 350 3 4.20. , , 4.2. 4.2
6、3 50 - 4.14 1 3. 4.21. . 4.24 4.18 4.15. 4.25 , 1, 1 100 80 % 5 1 , 10 90 . EN 158512013 3 4.26 1 , , . . , , . , , , , . . 4.27 1, = 10 /3 1 10 /3 4.24. 1 330 370 1 5.14. 4.24. , . 1, afl, /3, bafl100max , (1) max ( 330 370 ); 1, / ( = 312 /); 1 4.24, 2/, ( = 1930 2/) 2; b , . 18 . . 12 . 12 1 . 4.28 1, = 5,00 /3 4.27, 1,00 1, 200 3, 4.24. 1 5,00 /3. 4 , . . . 4.29 1, = 2 /3 4.27, 20 1, 10 3. ( ) , 4.16, . 1 2 /3. 4 , . . 3 . N 158512013 4 5 , , . . , , (, 15
