1、 . (ISO 12779:2011, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)ISO 127792014, - 1.092 . - 1.22009 . , . , , -, 1 - ( ) - , 52 (-)3 , (- 30 2014 . 68-) : ( 3166) 00497 ( 3166) 00497 AMBYKGMDRU -4 1 2014 . 858- ISO 127792014 - 1 2016 .5 ISO 12779:2011 Lactose Dete
2、rmination of water content Karl Fischer method (. . ). SC 5 - ISO/TC 34 (ISO). (en). , , . (IDT)6 - , - . () - . , , 2015 -, II ISO 127792014 . Lactose. Determination of water content. Karl Fischer method 201601011 - (KF).2 . 40 , , - (3.1).3 - , . - .3.1 (KF)1) KF , (3.2) -. (3.2) -, - (,) . KF, -
3、. (3.2) , (, ) - (, ). - (3.3) (3.5). , 40 .1)Hydranal-Titrant 2/Hydranal-Solvent Sigma-Aldrich apuraTitrant 2/apuraSolvent Merck . Hydranal-Composite 2 Sigma-Aldrich apuraCombiTitran 2 Merck . .1 ISO 127792014 3.2 KF (3.1) 2 /3. KF 5 /3 - 5 3(4.1).3.3 - KF 3.1.3.4 , w(H2O) = 10 / 10 /.3.5 (CH3OH),
4、0,05 % .4 . .4.1 . - 10 3. KF 5 /3 53(. 3.2). 40 - , .4.2 , (40 c177 5) , , (4.1). .4.3 , 1 0,1 .4.4 , 5 10 3, (3.4).4.5 , .5 . . , . 1.6 6.1 6.1.1 / () - (4.1). (4.1) 30 3- (3.3) . 5 ( ). (6.5) 40 , - - (4.2) 40 . -.2 ISO 1277920146.1.2 qV,3 , qv=Vtd, (1) Vd (3.2), (6.1.1), 3;t , . qm, , qm=Vtdec21
5、5c114, (2) c114e (6.2) (3.2), - (6.1.1), 1 3.6.2 KF6.2.1 c114e - (6.2.2) . (4.1) 30 3- (3.3) (. 6.1.1) -. , . (6.5) 40 , - . 40 (4.2). (4.4) 0,60,9 (3.4) (69 ). , 0,1 . - , . - 0,60,9 . -. (- ) . - . , , -. , 0,1 . , . (i = 1 5) miHO,2 0,1 . , , 10 , 6.3. (i = 1 5) Veic114, . -. - , 30 3-, - , .6.2.
6、2 c114e, i,13, c114e, i=mwVieiHO, HO,22c215sc114,(3) miHO,2 , i- (6.2.1), ;wsHO2, , /;Veic114, (3.2), i- (6.2.1), 3.3 ISO 1277920146.2.3 c114e,13, (i = 1 5) (6.2.1) c114e=c114eii,.c61c229155(4)6.2.4 .6.3 -, 10 , , (6.1).6.4 .6.5 (4.1) 30 3- (3.3). KF (3.1) - (4.2) 40 . (4.5) 0,3 (6.4). - . 0,1 . . ,
7、 0,1 . m, 0,1 . V. - -. (. 7.1), t. - . i- -mi, Vi ti.7 7.1 .7.1.1 wHO2, , :wVq tmVeHO2c61c215c45c215c215(),c114100 (5)wVqtmemHO2c61c215c45c215c114100, (6) V (6.5), 3;qV (6.1.2), 3/;qm (6.1.2), ;t (6.5), ;c114e (6.2.3), 1 3;m , (6.5).7.1.2 n , i- wHO,2i, , :4 ISO 127792014wHO,2i=()VqtmiVieic45c215c215c114c215100, (7)wHO,2i=Vqtmie miic215c45c215c114c215100, (8) Vi i- (6.5), 3;ti i- (6.5), ;mi i- (6.5), ;i , i =1n.7.1.3 wHO2, , - n , wHO2=wniinHO2,.c61c2291(9)7.2
