1、 () . (ISO 20837:2006, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)ISO 208372013, - 1.092 . - 1.22009 . , . , , -, 1 - - ( )2 (-)3 , (- 14 2013 . 44) : ( 3166) 00497 ( 3166) 00497 AMBYKGMDRUUZ -4 ISO 20837:2006 Microbiology offood and animal feeding stuffs Polyme
2、rase chain reaction (PCR) for the detection of food-bornepathogens Requirements for sample preparation for qualitative detection ( - . () . -). CEN/TC 275 -. ISO TC 34/SC 9 - ISO/TC 34 (ISO). - . (en). , , . (IDT)5 22 2013 . 2133- ISO 208372013 12015 .6 II ISO 208372013 - , - . () - . , , 2014 -, II
3、I ISO 2083720131 12 13 .14 25 , 26 2 () ().4 B () .5 () .7 .8IV ISO 208372013 () ( - ):- ;- () ;- ( );- ;- . , , . .V ISO 208372013 () . Microbiology of food and animal feeding stuffs.Polymerase chain reaction (PCR) for the detection of food-borne pathogens.Requirements for sample preparation for qu
4、alitative detection 20150701 , . - , . - .1 - , . . , - , A, - B. , .2 . . ( ).ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) forthe detection of food-borne pathogens General requirements and definitions ( . () . )3 3.1 , . , , , - .1 ISO 208372013 - ,
5、. , , - , - - .3.2 , - , .3.3 , - , . . -, , - .4 - ISO 22174, 6.3.5 , . A , ISO 22174.6 6.1 - . , - , , , , , . , , , , . - ( ). , , -, , , , . . -, , , -, , . - . , - , .6.2 6.2.1 6.2.1.1 . , - :a) (, ) - ;b) (, ), ;2 ISO 208372013c) - ;d) ;e) (, -(-)/TA (-TA ) -). , - , () (). , -/ - - . , , . (
6、- 10 ). - . (, , ), . - ( Mycobacterium spp.) , . , - , , .6.2.1.2 , , -, , . , - , -. . - . - () (. 1). ( ) - , -. . - . , , .6.3 ISO 22174, 9.3 1.3 ISO 208372013 A() () A.1 (-). A.1 () Salmonella spp. 6Staphylococcus aureus 7Bacillus cereus 8Clostridium perfringens 9Campylobacter spp. 10Yersinia enterocolitica 11Listeria monocytogenes 12Escherichia coli O157 13Shigella spp. 164 ISO 208372013 () .1 .2 1. , - Salmonella , -