GOST ISO 22119-2013 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and defi.pdf

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1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) ISO 22119 2013 () (ISO 22119:2011, IDT) ISO 221192013 II , 1.092 . 1.22009 . , . , , , 1 - ( ) 2 ( 335) 3 , ( 442013 14 2013 . 442013) : ( 3166) 00497 ( 3166) 00497 AM BY KZ KG MD - RU UZ 4 ISO 22119:2011 Microbiology of

2、 food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions ( . () . ). (en). CEN/TC 275 ISO TC 34/SC 9 ISO/TC 34 (ISO). , , . . (IDT) 5 22 2013 . 2132- ISO 221192013 1 2015 . 6 ISO 221192013 III , . () . ,

3、, 2014 , ISO 221192013 IV () , . , . . . ISO 221192013 1 () Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions 20150701 1 (). ( ) . , , . , , . . 2 . . ( ). ISO 20838 Microbiology o

4、f food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods ( () ) ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of fo

5、od-borne pathogens General requirements and definitions ( () ) 3 . 3.1 : , , . 1 , . . ISO 221192013 2 2 ISO 20838 . 3.2 -: , . 3.3 - : ( ) , . : 2. 3.4 : ( ) , . 3.5 : ( ) , / (). 3.6 : , , , . 3.7 5-3- : , , , 5-3. 5-3- . , , Taq-, Tth TFL-. 3.8 : . , , . 3.9 : , 5-3- . 1. ) ) ) 1 . 2 (). 3 . 4 .

6、1 ISO 221192013 3 3.10 : , , , . 2. ) ) 1 . 2 . 3 . 2 3.11 : . : , , 5- 3-, ; , . 3. ) ) , 1 . 2 (). 3 . 3 3.12 : , , , , . 3.13 : , . 1 , , . 2 , . ISO 221192013 4 3.14 : , , . , . 3.15 , : , . 3.16 , : , . 3.17 : , . 4 4.1 : a) ; b) ; c) ; d) ; e) . , . 4.2 4.2.1 , . , , . . , 5-3- - . , . -. 3- , . 4.2.2 , . , , , , . , - , , , -. 3- , . 4.2.3

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