1、 550272012/ISO/TS10272-3:2010 Campylobacter spp. 3 ISO/TS 10272-3:2010Microbiology of food and animal feeding stuffs Horizontal method for detectionand enumeration of Campylobacter spp. Part 3: Semi-quantitative method(IDT) 27 2002 . 184- , 1.02004 . 1 - ( ) , - 42 335 - 3 24 2012 . 555-4 / 10272-3:
2、2010 . Campylobacter spp. 3. (ISO/TS 10272-3:2010Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration ofCampylobacter spp. Part 3: Semi-quantitative method) ISO/TS 10272-3:2010/Cor.1:2011. ( 3) . - -, 5 , - . () . - , , 2013 , - - II 550272012/ISO/TS 10272-3
3、:20101 .12 13 24 .25 26 .37 .48 49 410 711 8 A () 9 B () .10 () ( ) 1516III 550272012/ISO/TS 10272-3:2010 Campylobacter spp. 3 Microbiology of food and animal feeding stuffs. Horizontal method for detection and enumeration of Campylobacter spp.Part 3. Semi-quantitative method 201401011 Campylobacter
4、spp. :- ;- . .2 -. ( ). 6887 ( ) . , - (ISO 6887 Microbiology of food and animal feeding stuffs Preparation of test samples, initialsuspension and decimal dilutions for microbiological examination (all part) 7218 . - (ISO 7218 Microbiology of food and animal feedingstuffs General requirements and gu
5、idance for microbiological examinations)/ 11133-1 . -.1.- (ISO/TS 11133-1 Microbiology of foodand animal feeding stuffs Guidelines on preparation and production of culture media Part 1: Generalguidelines on quality assurance for the preparation of culture media in the laboratory)/ 11133-2:2003 . . 2
6、. - (ISO/TS 11133-2:2003 Microbiology of food andanimal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practicalguidelines on performance testing of culture media)1 550272012/ISO/TS 10272-3:2010 3 .3.1 Campylobacter (Campylobacter) ( Campylobacter ): , -, 41,5 C, 25
7、 C, , , - , . Campylobacter jejuni C. coli. - (C. lari, C. upsaliensis ).3.2 (semi-quantitative determination) (Campylobacter ): Campylobacter , , .4 4.1 Campylobacter spp. , 4.24.4 (. A.1).4.2 - ( ) . 37 C 46 41,5 C (44 c177 4) .4.3 , 4.2, , , (mCCD ), , 41,5 C (44 c177 4) , Campylobacter spp.4.4 ,
8、 Campylobacter spp., - , . Campylobacter spp. - .5 5.1 . 7218,/ 11133-1 / 11133-2. -, B.5.2 : . B.1.5.3 : , (mCCD ). B.2.2 550272012/ISO/TS 10272-3:20105.4 5.4.1 . B.3.5.4.2 . B.4.5.4.3 . B.5.5.4.4 , 3 % ( )5.4.5 . B.6.5.4.6 -. B.7.5.4.7 30 .5.4.8 . B.8.6 (. 7218) , -, .6.1 ( ) (). 7218.6.2 , , 37 C
9、 55 C.6.3 , (25 c177 1) C, (37 c177 1) C (41,5 c177 1) C.6.4 , (37 c177 1) C.6.5 , 47 C 50 C.6.6 pH-, 0,1 . pH 25 C.6.7 , , 18 c180 180 9 c180 180 , 13 c180 75 , / .6.8 , , 90 100 .6.9 , , , 1 3103, 0,1 3, 835 A 1, - , 7712 2.6.10 , , - .6.11 , - - -, 3, ,- . - (. 9.5.6).6.12 , , , .6.13 ( - Campylo
10、bacter spp.).6.14 , : (5 c177 2) %, (10 c177 3) %, c163 10 %, . , / , 350 3, -, , . 1 - ; ,3 550272012/ISO/TS 10272-3:2010 , . - . 2 , , , - , 20 .7 . - . , . , . , , . , Campylobacter spp. , - , , (3 c177 2) C . - .8 ISO 6887,- . ISO 6887, -, .9 9.1 . A.1.9.2 , 9.2.1 x x 3( 15 15 3) ( 8) (120 3) (5
11、.2) . .9.2.2 90 3 (9.2.1) 100 3. - 10 . 101.9.2.3 10 3 (9.2.1) . 100. (9.2.4) 93 . 1 .9.2.4 10- (, 104)100(9.2.3), 1,0 3 , 9,0 3 . - 1,0 3, 9,0 3. - 101,102.9.3 (9.2.2, 9.2.3 9.2.4) (6.14) 37 C 4 6 , 41,5 C (44 c177 4) .9.4 9.4.1 , (9.3), (6.11) mCCD (5.3).9.4.2 (9.4.1) 41,5 C (6.14).9.4.3 (44 c177 4)
