1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 10 31 http:/www.kats.go.krKS I ISO 20079 (Lemna minor) KS I ISO 20079： 2006 (2011 )I ISO 20079： 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) ： ：2006 10 31 ：2011 12 26 2011-0623 ： ： ( 02- 509-7270) (http:/www.kats.go.kr). 10 5 , . ICS 13.060.70
2、KS I ISO (Lemna minor) 20079： 2006(2011 ) Water qualityDetermination of the toxic effect of water constituents and waste water on duckweed (Lemna minor)Duckweed growth inhibition test 2005 1 ISO 20079 Water quality Determination of the toxic effect of water constituents and waste water on duckweed(L
3、emna minor) Duckweed growth inhibition test . . . . . 1. , (Lemna minor) . 2. . , . ( .) . KS M ISO 5667 16 16： ISO 10260 Water quality Measurement of biochemical parameters Spectrometric determination of the chlorophyll-a concentration 3. . 3.1 (axenic cultures) , 3.2 (calculation parameters) , , .
4、 3.3 (chlorosis) ( ) 3.4 (colony) I ISO 20079： 2006 2 3.5 (control batch) 3.6 (control medium) / 3.7 (dilution water) 3.8 (doubling time) 2 3.9 (effective concentration) , x % (ECx) EC “EC(r)” . , EC(r)( ) 3.10 (frond) . ( , ), . 3.11 (frond area) 3.12 (frond number) 3.13 (growth) . 3.14 (growth-rat
5、e) , . 2 , (12.1.2 ) . 3.15 (inoculum) ( ) 3.16 (necrosis) ( , ) 3.17 (nutrient medium) , 3.18 (observation parameters) , , , , ( , , , ). 3.19 (pre-culture) 3.20 (root) (Lemna) 3.21 (stock culture) (Lemna) , , . 3.22 (test batch) 3.23 (test medium) , / 3.24 (test sample) ( , , , ) ( , , , .) 4. 7 L
6、emna minor . ( , ) , . I ISO 20079： 2006 3 , , x % , EC(r)x . 5. , , , / , , (KS M ISO 5667 16 ). . 6. . 6.1 ( , , ). 150 mL( 2/3 , , 100 mL ) . 6.2 . 6.3 , 6.4 665 nm 750 nm 6.5 . 6.6 pH 6.7 6.8 ( , , , ) 6.9 . 6.10 6.11 . 7. 7.1 10 S/cm 7.2 , c(HCl)=0.1 mol/L 7.3 , c(NaOH)=0.1 mol/L 7.4 C6H12O67.5
7、 A 7.6 (STEINBERG) ( 1 ) , . A . I ISO 20079： 2006 4 1 pH ( STEINBERG) (Altenburger ) mg/L mmol/L KNO3101.12 350.00 3.460 Ca(NO3)2 4H2O 236.15 295.00 1.250 KH2PO4136.09 90.00 0.660 K2HPO4174.18 12.60 0.072 MgSO4 7H2O 246.37 100.00 0.410 g/L mol/L H3BO361.83 120.00 1.940 ZnSO4 7H2O 287.43 180.00 0.63
8、0 Na2MoO4 2H2O 241.92 44.00 0.180 MnCl2 4H2O 197.84 180.00 0.910 FeCl3 6H2O 270.21 760.00 2.810 EDTA Disodium-dihydrate 372.24 1 500.00 4.030 7.6.1 (stock solution) ( 2 3 ) . 10 , . 2 (stock solution)( ) (50 ) g/L (stock solution) 1： KNO317.50 KH2PO404.50 K2HPO400.63 (stock solution) 2： MgSO4 7H2O 0
9、5.00 (stock solution) 3： Ca(NO3)2 4H2O 14.75 3 (stock solution)( ) (1 000 ) mg/L (stock solution) 4： H3BO30 120.00 (stock solution) 5： ZnSO4 7H2O 0 180.00 (stock solution) 6： Na2MoO4 2H2O 0 044.00 (stock solution) 7： MnCl2 4H2O 0 180.00 (stock solution) 8： FeCl3 6H2O 0 760.00 EDTA Disodium-dihydrate
10、 1 500.00 (stock solution) 2 3 47 ( ). I ISO 20079： 2006 5 121 20 (0.2 m) . (stock solution) 8 (0.2 m) . 7.6.2 STEINBERG (stock solution) 1, 2 3( 2 ) 20 mL 900 mL (7.1) . (stock solution) 4, 5, 6, 7 8( 3 ) 1 mL . pH 5.50.2 NaOH (7.3) HCl(7.2) . (7.1) 1 000 mL . , , . , (121 20 ) (stock solution) 8 .
11、 7.6.3 10 STEINBERG (stock solution) 1, 2 3( 2 ) 20 mL 30 mL (7.1) . (stock solution) 4, 5, 6, 7 8( 3 ) 1.0 mL . (7.1) 100 mL . (stock solution) , , . , (121 20 ) (stock solution) 8 . pH( ) 5.50.2 . , , APHA . APHA( , EDTA , A ) STEINBERG APHA . 9.2 , . APHA A . ( ) , OECD ( SIS) ( A ). . 7.7 7.7.1
12、3,5 3,5 99 % 7.7.2 KCl 8. , (Lemna minor)( ) . . 9. 9.1 . (1 %) . , 1 % ( A ). , . 9.2 710 . 1 . . 2. APHA , ( A ) . . I ISO 20079： 2006 6 7 7 ( , r 0.275/ 2.5 ). , , . . 3 cm . , ( ) 50 % . . 10. 10.1 KS M ISO 5667 16 . (7.2) (7.3) pH (0.2) pH . pH . pH 5 pH 9 . pH 5 8 pH . pH pH . 10.2 EC(r )x EC(
13、r)x , EC(r)x . 5 . EC(r)x 1 EC(r)x , EC(r)x . 3 0 % 100 % . , . 10.3 10.3.1 EC(r)x EC(r)x (KS M ISO 5667 16 ) , 3 , 6 . 10.3.2 EC(r)x 4 L) , pH . (7.1) 1 000 mL . NaOH (7.3) HCl(7.2) pH 8.30.1 . . A.1.3 ( APHA ) 97 % 1 000 mL 10 mL I ISO 20079： 2006 12 3 (stock solution)(A, B C) . ( , 0.5 mm) , pH 2
14、0 . 100 . , (0.2 m ) . . A.2 OECD SIS A.2.1 (stock solution) A.2 OECD TG 221(2004 4) (stock solution) g/L (1) mg/L (stock solution) MgSO4 7H2O 15 75 II NaNO38.5 85 I CaCl2 2H2O 7.2 36 III Na2CO34.0 20 IV KH2PO41.34 13.4 I H3BO31.0 1.0 V MnCl2 4H2O 0.2 0.2 Na2MoO4 2H2O 0.010 0.010 V ZnSO4 7H2O 0.050
15、0.050 CuSO4 5H2O 0.005 0.005 V Co(NO3)2 6H2O 0.010 0.010 Na2EDTA 0.28 1.4 VI(2) FeCl3 6H2O 0.168 0.84 VI MOPS(buffer)(3) 490 490 VII pH NaOH (7.3) HCl(7.2) 6.50.2 . (121 , 15 ) ( 0.2 m) (stock solution) I V . (stock solution) VI( VII)( , .) (1) (2) (3) pH MOPS ( ) A.2.2 SIS 900 mL (7.1) (stock solut
16、ion) . 10 mL (stock solution) I 5 mL (stock solution) II, III, IV, VI 1 mL (stock solution) V 1 mL (stock solution) VII( ) NaOH (7.3) HCl (7.2) pH . (stock solution) , , . I ISO 20079： 2006 13 (stock solution) VI( VII) (121 20 ) . A.3 A.3.1 (stock solution) A.3 Jungnickel(1986) ( Jungnickel P ) mg/L
17、 mmol/L KNO3101.12 809.000 8.000 0 Ca(NO3)2 4H2O 236.15 236.000 1.000 0 KH2PO4136.09 204.000 1.500 0 MgSO4 7H2O 246.37 246.000 1.000 0 H3BO3061.83 000.310 0.050 0 MnCl2 4H2O 197.84 002.570 0.130 0 Na2MoO4 2H2O 241.92 000.097 0.000 4 FeCl3 6H2O 270.21 038.900 0.140 0 EDTA disodium-dihydrate 372.24 04
18、8.400 0.130 0 Glucose monohydrate 10 000 9 000 A.4 (stock solution)(20 ) (stock solution) 20 g/L 1 KNO316.18 1 KH2PO404.08 2 MgSO4 7H2O 04.92 3 Ca(NO3)2 4H2O 04.72 mg/L 4 H3BO306.20 4 Na2MoO4 2H2O 01.94 4 MnCl2 4H2O 51.40 5 FeCl3 6H2O 778 5 EDTA disodium-dihydrate 968 (7.1) , 121 20 . (42) 3 . A.3.2
19、 50 mL (stock solution) 1, 2, 3, 4, 5 500 mL (7.1) . 10 g (7.4) 9 g (7.5) . (7.1) 1 000 mL . 121 20 . , , . (stock solution) . I ISO 20079： 2006 14 B() (LID) B.1 (D) (LID) , . D . 1/4 (25 % ) D=4 . B.2 B.2.1 ( D) D . , D=1 . B.2.2 (LID) (LID) 10 % . B.3 , D . , . . 10 % , . B.4 LID . , B.1 . . B.1 1
20、00 mL , . , (7.1) 100 mL , . 10 % . 10 % (LID) D . D (： ： , , ) . I ISO 20079： 2006 15 B.1 LID D L/L mL mL 0 001 0.9(1) 90.00 10 00.00 0 002 0.500 0 50.00 10 40.00 0 003 0.333 3 33.33 10 56.67 0 004 0.250 0 25.00 10 65.00 0 006 0.166 7 16.67 10 73.33 0 008 0.125 0 12.50 10 77.50 0 012 0.083 3 08.33
21、10 81.67 0 016 0.062 5 06.25 10 83.75 0 024 0.041 7 04.17 10 85.83 0 032 0.031 3 03.13 10 86.87 0 048 0.020 8 02.08 10 87.92 0 064 0.015 6 01.56 10 88.44 0 096 0.010 4 01.04 10 88.96 0 128 0.007 8 00.78 10 89.22 0 192 0.005 2 00.52 10 89.48 0 256 0.003 9 00.39 10 89.61 0 384 0.002 6 00.26 10 89.74 0 512 0.002 0 00.20 10 89.80 0 768 0.001 3 00.13 10 89.87 1 024 0.001 0 00.10 10 89.90 ： 10 , ： 100 mL (1) D =1 . B.5 LID LID 3 6
