1、BRITISH STANDARD BS EN 20-2:1993 Wood preservatives Determination of the protective effectiveness against Lyctus brunneus (Stephens) Part 2: Application by impregnation (laboratory method) The European Standard EN20-2:1993 has the status of a British StandardBSEN20-2:1993 This British Standard, havi
2、ng been prepared under the directionof the Technical SectorBoard for Building and Civil Engineering, was publishedunder the authority ofthe Standards Board and comesinto effect on 15June1993 BSI 01-2000 The following BSI references relate to the work on this standard: Committee reference B/515 Draft
3、 for comment 90/55125DC ISBN0580214176 Cooperating organizations The European Committee for Standardization (CEN), under whose supervision this European Standard was prepared, comprises the national standards organizations of the following countries: Austria Oesterreichisches Normungsinstitut Belgiu
4、m Institut belge de normalisation Denmark Dansk Standardiseringsraad Finland Suomen Standardisoimisliito, r.y. France Association franaise de normalisation Germany Deutsches Institut fr Normung e.V. Greece Hellenic Organization for Standardization Iceland Technological Institute of Iceland Ireland N
5、ational Standards Authority of Ireland Italy Ente Nazionale Italiano di Unificazione Luxembourg Inspection du Travail et des Mines Netherlands Nederlands Normalisatie-instituut Norway Norges Standardiseringsforbund Portugal Instituto Portugus da Qualidade Spain Asociacin Espaola de Normalizacin y Ce
6、rtificacin Sweden Standardiseringskommissionen i Sverige Switzerland Association suisse de normalisation United Kingdom British Standards Institution Amendments issued since publication Amd. No. Date CommentsBSEN20-2:1993 BSI 01-2000 i Contents Page Cooperating organizations Inside front cover Natio
7、nal foreword ii Foreword 2 Text of EN 20-2 3 National annex NA (informative) Committees responsible Inside back cover National annex NB (informative) Cross-references Inside back coverBSEN20-2:1993 ii BSI 01-2000 National foreword This Part of BSEN20 has been prepared under the direction of the Tech
8、nical Sector Board for Building and Civil Engineering and is the English language version of EN20-2 Wood preservatives Determination of the protective effectiveness against Lyctus brunneus (Stephens) Part2: Application by impregnation (laboratory method), published by the European Committee for Stan
9、dardization (CEN). EN20-2 was produced as a result of international discussion in which the United Kingdom took an active part. BS EN 20 consists of the following Parts: Part 1: Application by surface treatment (laboratory method); Part 2: Application by impregnation (laboratory method). Part 1 is i
10、dentical with EN20-1:1992 and superseded BS5217:1975 which has been withdrawn. CAUTION. Attention is drawn to the Health and Safety at Work etc. Act1974, and the need for ensuring that the method specified in this Part of BSEN20 is carried out with suitable precautions. The procedure described in th
11、is Part of BSEN20 is intended to be carried out by appropriately qualified and experienced persons or other suitably trained and/or supervised personnel. Attention is drawn to the precautions given in the introduction,5.2.5 and5.3.4. A British Standard does not purport to include all the necessary p
12、rovisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, theEN title
13、page, pages 2 to 14, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 20-2 April 1993 UDC 6
14、74.048.4:620.193.8 Descriptors: Wood, wood preservatives, pesticides, insecticides, pest control, lyctus, prevention, determination, effectiveness, laboratory tests English version Wood preservatives Determination of the protective effectiveness against Lyctus brunneus (Stephens) Part2: Application
15、by impregnation (laboratory method) Produits de prservation du bois Dtermination de lefficacit protectrice vis-vis de Lyctus brunneus (Stephens) Partie2: Application par traitement en profondeur (Mthode de laboratoire) Holzschutzmittel Bestimmung der vorbeugenden Wirkung gegenber Lyctus brunneus (St
16、ephens) Teil2: Anwendung durch Volltrnkung (Laboratoriumsverfahren) This European Standard was approved by CEN on1993-04-02. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard wi
17、thout any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language
18、 made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, It
19、aly, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. CEN European Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1993 Copyright reserved to CEN members Ref.
20、 No. EN 20-2:1993 EEN20-2:1993 2 BSI 01-2000 Foreword This Part of this European Standard has been drawn up by the “Lyctus” Expert Group of CEN/TC38 “Durability of wood and wood-based products”, with AFNOR as secretariat. This Part of EN20 together with EN20-1 replaces EN20:1974. This Part of EN20 i
21、s required to enable effectiveness assessments of preservatives which are intended to be applied by impregnation. This Part of this European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October1993, and conf
22、licting national standards shall be withdrawn at the latest by October1993. This Part of this European Standard was adopted by CEN and in accordance with the CEN/CENELEC Internal Regulations the following countries are bound to implement this Part of EN20: Austria, Belgium, Denmark, Finland, France,
23、 Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. Contents Page Foreword 2 Introduction 3 1 Scope 3 2 Normative reference 3 3 Definitions 3 4 Principle 3 5 Test materials and apparatus 4 6 Sampling 4 7 Test specimens
24、5 8 Procedure 5 9 Validity of test 7 10 Expression of results 7 11 Test report 7 Annex A (informative) Example of a test report 9 Annex B (informative) Technique for culturingLyctus brunneus 11 Annex C (informative) Principal parasites andpredators of Lyctus 14 Annex D (informative) Bibliography 14
25、Figure B.1 Checking for the presence of sufficient starch content in European oak using Lugol reagent sufficient starchcontent 12 Figure B.2 Checking for the presence of sufficientstarch content in European oak usingLugol reagent insufficient starch content 13 Figure B.3 Last ventral segment of the
26、abdomen of Lyctus brunneus for the identification of sex 13 Table A.1 Results 10EN20-2:1993 BSI 01-2000 3 Introduction This Part of EN20 describes a laboratory method of test which gives a basis for assessment of the effectiveness of a wood preservative, against Lyctus brunneus. It allows the determ
27、ination of the concentration at which the preservative completely prevents the development of infestation from egg-laying in fully impregnated wood of susceptible species. The species Lyctus brunneus is chosen because of its particular practical relevance and because it can be used easily in laborat
28、ory tests. The method can be used with other lyctid species, but the results may not be comparable with those obtained with Lyctus brunneus. The test specimens are enriched with a defined nutrient solution, before exposure to egg-laying, in order to ensure uniformity of nutrient quality of test spec
29、imens between different laboratories. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further recommended that results from this test shou
30、ld be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chem
31、ical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel. 1 Scope This Part of EN20 specifies a method for the det
32、ermination of the protective effectiveness or the toxic values of a wood preservative against infestation by Lyctus brunneus (Stephens) in wood which has been treated previously by full impregnation. This method is applicable to: water-insoluble chemicals which are being studied as active insecticid
33、es, or, organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates. This method is not applicable to water-based perservatives. NOTEThis method may be used in conjuction with ageing procedures, which do not remove the added nutrient. 2 Normative reference This Eur
34、opean Standard incorporates by dated or undated reference, provisions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publication
35、s apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies. ISO 3696:1987, Water for analytical laboratory use Specification and test methods. 3 Definitions For the purposes of this Part of
36、 EN20, the following definitions apply. 3.1 representative sample a sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled 3.2 supplier the sponsor of the test 4 Principle Depending on the test being carried out ei
37、ther: a set of test specimens of a susceptible wood species is impregnated with a nutrient solution and then impregnated with a solution of the preservative; or if toxic values are to be determined, several sets of test specimens of a susceptible wood species are impregnated with a nutrient solution
38、 and then impregnated with a series of solutions in which the concentration of preservative is ranged in a given progession. The treated test specimens are exposed to adult Lyctus brunneus and the resulting attack compared with that in untreated controls. If the preservative has been prepared in the
39、 laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared with that in solvent or diluent treated controls.EN20-2:1993 4 BSI 01-2000 5 Test materials and apparatus 5.1 Biological material Lyctus brunneus (Stephens), insects emerged from cultures not
40、 more than48h before use in the test, reared for at least two generations on non-enriched oak or no more than three generations on enriched oak. NOTEThe culturing of Lyctus brunneus requires care in order to obtain a regular supply of adults which have not already laid eggs. The culturing technique,
41、 which experience has shown to be suitable, is described inAnnex B. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens treated with solutions. NOTEParaffin wax with a setting point of52 C to54 C has been found to be suitable. 5.2.2 Filter paper, ordinary qua
42、lity medium-fast grade. 5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Lyctus and insoluble in the product under test. NOTESodium carboxymethyl cellulose, food grade, has been found suitable. 5.2.4 Water, complying with grade3 of ISO3696. 5.2.5 Solvent or diluen
43、t, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION. Do not use benzene or other solvents which pose a health risk. 5.2.6 Peptone, prepared as
44、an enzymatic hydrolysate of meat. 5.2.7 D( + )glucose 5.2.8 Fine cloth, of cotton or linen, with a mesh aperture of less than0,3mm. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (26 1) C, and at relative humidity(75 5)%. 5.3.2 Conditioning chamber, well ventilated, contr
45、olled at (20 2) C and relative humidity(65 5)%. NOTEThe conditioning of specimens may be carried out in the laboratory work area (see5.3.4) provided that this has the conditions specified for the conditioning chamber (see5.3.2). 5.3.3 Drying chamber, well ventilated, controlled at(30 2) C. 5.3.4 Lab
46、oratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION. It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.5 Testing chamber, with conditions identi
47、cal to those of the culturing chamber (see5.3.1). 5.3.6 Treatment vessels, of material that does not react with the preservative under test; for example of glass for organic products. 5.3.7 Vacuum vessel(s), fitted with stopcocks. 5.3.8 Vacuum pump, fitted with a pressure gauge and capable of mainta
48、ining a pressure of700Pa 1) . 5.3.9 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test. 5.3.10 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to ensure
49、the safety of the operator. 5.3.11 Test containers, suitable for holding the test specimens and of material resistant to the solvents used. NOTEJars of approximately60mm diameter and100mm height have been found to be suitable. 5.3.12 Drying vessels(s), capable of holding sets of five test specimens (7.4), provided with a close-fitting cover and containing supports that will give minimum contact with treated test specimens to be placed on them. The vessels and supports shall be of a material that does not re
