1、BSI Standards PublicationBS EN ISO 17234-1:2015Leather Chemical tests forthe determination of certainazo colorants in dyed leathersPart 1: Determination of certain aromaticamines derived from azo colorantsBS EN ISO 17234-1:2015 BRITISH STANDARDNational forewordThis British Standard is the UK impleme
2、ntation of EN ISO17234-1:2015. It supersedes BS EN ISO 17234-1:2010 which iswithdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee TCI/69, Footwear, leather and coated fabrics.A list of organizations represented on this committee can beobtained on request to its secret
3、ary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2015. Published by BSI StandardsLimited 2015ISBN 978 0 580 78779 9ICS 59.140.30Compliance with a British Standard cannot con
4、fer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 30 April 2015.Amendments issued since publicationDate Text affectedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 17234-1 April 2015 ICS 59.140.30 Sup
5、ersedes EN ISO 17234-1:2010English Version Leather - Chemical tests for the determination of certain azo colorants in dyed leathers - Part 1: Determination of certain aromatic amines derived from azo colorants (ISO 17234-1:2015)Cuir - Essais chimiques pour le dosage de certains colorants azoques dan
6、s les cuirs teints - Partie 1: Dosage de certaines amines aromatiques drives des colorants azoques (ISO 17234-1:2015) Leder - Chemische Prfungen zur Bestimmung bestimmter Azofarbstoffe in gefrbten Ledern - Teil 1: Bestimmung bestimmter aromatischer Amine aus Azofarbstoffen (ISO 17234-1:2015) This Eu
7、ropean Standard was approved by CEN on 14 February 2015. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical reference
8、s concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN mem
9、ber into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, Fr
10、ance, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES
11、KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 17234-1:2015 EBS EN ISO 17234-1:2015EN ISO 17234-1:2015 (E) 3 Foreword This document (EN I
12、SO 17234-1:2015) has been prepared by IULTCS “International Union of Leather Technologists and Chemists Societies“ in collaboration with Technical Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI. This European Standard shall be given the status of a national standard, either b
13、y publication of an identical text or by endorsement, at the latest by October 2015, and conflicting national standards shall be withdrawn at the latest by October 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or C
14、ENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 17234-1:2010. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria,
15、 Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, S
16、witzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 17234-1:2015 has been approved by CEN as EN ISO 17234-1:2015 without any modification. BS EN ISO 17234-1:2015ISO 17234-1:2015(E)IULTCS/IUC 20-1:2015(E) ISO 2015 All rights reserved iiiContents PageForeword iv1 Scope . 12 N
17、ormative references 13 General 14 Principle 25 Safety precautions 36 Apparatus . 37 Reagents 48 Sampling and preparation of samples . 59 Procedure. 59.1 Degreasing 59.2 Reductive cleavage . 59.3 Liquid-liquid extraction 59.4 Check of the analytical system . 610 Chromatographic analyses . 611 Calibra
18、tion 612 Evaluation 612.1 Calculation of amine in the sample . 612.2 Reliability of the method 713 Test report . 7Annex A (informative) Chromatographic analyses 8Annex B (informative) Reliability of the method 12Annex C (informative) Assessment guide Interpretation of analytical results .13Bibliogra
19、phy .15BS EN ISO 17234-1:2015ISO 17234-1:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each me
20、mber body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International E
21、lectrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different type
22、s of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be h
23、eld responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents). Any trade name used in this document is informat
24、ion given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the f
25、ollowing URL: Foreword - Supplementary informationISO 17234-1 was prepared by the Chemical Test Commission of the International Union of Leather Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European Committee for Standardization (CEN) Technical Committee CE
26、N/TC 289, Leather, the secretariat of which is held by UNI, in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement). This method is technically similar to the method in IUC 20, which was declared an official method at the IULTCS Delegates meeting on 31st May
27、2003 in Cancun, Mexico.IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to further the advancement of leather science and technology. IULTCS has three Commissions, which are responsible for establishing international methods for the sampling and testi
28、ng of leather. ISO recognizes IULTCS as an international standardizing body for the preparation of test methods for leather.This second edition of ISO 17234-1 cancels and replaces the first edition (ISO 17234-1:2010), which has been technically revised to include two new aromatic amines from the Chi
29、nese Standard GB 20400 in Table 1. The layout for this part of ISO 17234 has been re-arranged and updated to be the same as ISO 17234-2.ISO 17234 consists of the following parts, under the general title Leather Chemical tests for the determination of certain azo colorants in dyed leathers: Part 1: D
30、etermination of certain aromatic amines derived from azo colorants Part 2: Determination of 4-aminoazobenzeneIULTCS/IUC 20-1:2015(E)iv ISO 2015 All rights reservedBS EN ISO 17234-1:2015INTERNATIONAL STANDARD ISO 17234-1:2015(E)Leather Chemical tests for the determination of certain azo colorants in
31、dyed leathers Part 1: Determination of certain aromatic amines derived from azo colorants1 ScopeThis part of ISO 17234 specifies a method for determining the use of certain azo colorants which can release certain aromatic amines.2 Normative referencesThe following documents, in whole or in part, are
32、 normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 2418, Leather Chemical, physical and mechanical and f
33、astness tests Sampling locationISO 3696:1987, Water for analytical laboratory use Specification and test methodsISO 4044, Leather Chemical tests Preparation of chemical test samplesISO 17234-2, Leather Chemical tests for the determination of certain azo colorants in dyed leathers Part 2: Determinati
34、on of 4-aminoazobenzene3 GeneralCertain azo colorants can release, by reductive cleavage of azo group(s), one or more of the following aromatic amines, which are listed in Appendix 8 of EU Regulation 1907/2006, Annex XVII of the European Parliament, and/or the Chinese Standard GB 20400-2006.Table 1
35、Aromatic amines listed in Appendix 8 of EU regulation 1907/2006, Annex XVII, and/or GB 204002006No. CAS number Index number EC number Substances1 92671 612072006 2021771 biphenyl-4-ylamine 4-aminobiphenyl xenylamine2 92875 612042002 2021991 benzidine3 95692 2024416 4-chloro-o-toluidine4 91598 612022
36、003 2020804 2-naphthylamine5a97563 611006003 2025912 o-aminoazotoluene 4-amino-2,3-dimethyla-zobenzene 4-o-tolylazo-o-toluidine6a99558 2027658 5-nitro-o-toluidine7 106478 612137009 2034010 4-chloroaniline ISO 2015 All rights reserved 1BS EN ISO 17234-1:2015ISO 17234-1:2015(E)No. CAS number Index num
37、ber EC number Substances8 615054 2104061 4-methoxy-m-phenylenedi-amine9 101779 612051001 2029744 4,4-methylenedianiline 4,4-diaminodiphenylmeth-ane10 91941 612068004 2021090 3,3-dichlorobenzidine 3,3-dichlorobiphenyl-4,4-ylenediamine11 119904 61203600-X 2043554 3,3-dimethoxybenzidine o-dianisidine12
38、 119937 612041007 2043580 3,3-dimethylbenzidine 4,4-bi-o-toluidine13 838880 612085007 2126588 4,4-methylenedi-o-tolui-dine14 120718 2044191 6-methoxy-m-toluidine p-cresidine15 101144 612078009 2029189 4,4-methylene-bis-(2-chloro-aniline) 2,2-dichloro-4.4-methyl-ene-dianiline16 101804 2029770 4,4-oxy
39、dianiline17 139651 2053709 4,4-thiodianiline18 95534 61209100-X 2024290 o-toluidine 2-aminotoluene19 95807 612099003 2024531 4-methyl-m-phenylenedi-amine20 137177 2052820 2,4,5-trimethylaniline21 90040 612035004 2019631 o-anisidine 2-methoxyaniline22b60093 611008004 2004536 4-aminoazobenzene23c95681
40、 612027000 2024400 2,4-xylidine2,4-dimethylbenzene-1-amine24c87627 61216100-X 2017587 2,6-xylidine2,6-dimethylbenzene-1-amineaThe CAS-numbers 97563 (No. 5) and 99558 (No. 6) are further reduced to CAS-numbers 95534 (No. 18) and 95807 (No. 19).bAzo colorants that are able to form 4-aminoazobenzene, g
41、enerate under the condition of this method aniline and/or 1,4-phenylenediamine. The presence of these colorants shall be tested using ISO 17234-2.cAdditional aromatic amines in the Chinese Standard GB 2040020064 PrincipleAfter degreasing, the leather sample is treated with sodium dithionite in an aq
42、ueous buffer solution (pH 6) at 70 C in a closed vessel. The amines released in the process of reductive cleavage are transferred to a t-butyl methyl ether phase by means of liquid-liquid extraction using Kieselgur columns. The t-butyl methyl ether extract is then concentrated under mild conditions
43、in a rotary vacuum evaporator and the residue is dissolved in a suitable solvent, depending on the method used to determine the amines.IULTCS/IUC 20-1:2015(E)Table 1 (continued)2 ISO 2015 All rights reservedBS EN ISO 17234-1:2015ISO 17234-1:2015(E)Determination of the amines is performed by means of
44、 high-peformance liquid chromatography (HPLC) using a diode array detector (DAD) or mass selective detector (HPLC/MS), capillary gas chromatography with a mass-selective detector (GC-MS) or by capillary electrophoresis with a diode array detector (CE/DAD), or qualitatively with thin layer chromatogr
45、aphy (TLC, HPTLC).The amines shall be identified by means of at least two different chromatographic separation methods in order to avoid any possible misinterpretations caused by interfering substances (such as position isomers of the amines to be identified) and hence any incorrect statements. Amin
46、e quantification shall be performed by HPLC/DAD or GC/MS.5 Safety precautions5.1 WARNING The aromatic amines listed in Clause 3 are classified as substances known to be or suspected to be human carcinogens.Any handling and disposal of this substance shall be in strict accordance with the appropriate
47、 national health and safety regulations.5.2 It is the users responsibility to use safe and proper techniques when handling materials in this test method. Consult manufacturers for specific details, such as material safety data sheets and other recommendations.5.3 Good laboratory practice should be f
48、ollowed. Wear safety glasses in all laboratory areas, and a dust respirator and single-use gloves while handling powder colorants and aromatic amines.5.4 Users should comply with any national and local safety regulations.6 ApparatusUsual laboratory equipment and, in particular, the following:6.1 Sui
49、table reaction vessel, of temperature-resistant glass with a gas-tight closure.6.2 Hot cabinet with sand bath (sea sand, 0,1 mm to 0,3 mm) or water bath with thermostat.6.3 Thermometer, 0,5 C accuracy at 70 C.6.4 Volumetric flasks, different volumes.6.5 Polypropylene or glass column 1)with 25 mm to 30 mm inner diameter and 140 mm to 150 mm length, glass filter at
