1、Microbiology of the food chain Preparation of test samples, initial suspension and decimal dilutions for microbiological examinationPart 1: General rules for the preparation of the initial suspension and decimal dilutionsBS EN ISO 6887-1:2017BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.
2、indd 1 15/05/2013 15:06EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 6887-1 April 2017 ICS 07.100.30 Supersedes EN ISO 6887-1:1999English Version Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1
3、: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017) Microbiologie de la chane alimentaire - Prparation des chantillons, de la suspension mre et des dilutions dcimales en vue de lexamen microbiologique - Partie 1: Rgles gnrales pour la prparation de l
4、a suspension mre et des dilutions dcimales (ISO 6887-1:2017) Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdnnungen und von Dezimalverdnnungen fr mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln fr die Herstellung von Erstverdnnungen
5、 und Dezimalverdnnungen (ISO 6887-1:2017) This European Standard was approved by CEN on 14 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration
6、. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by t
7、ranslation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Fi
8、nland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STA
9、NDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 6887-1:2017 ENational forewordThis
10、 British Standard is the UK implementation of EN ISO 6887-1:2017. It is identical to ISO 6887-1:2017. It supersedes BS EN ISO 6887-1:1999, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this co
11、mmittee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limited 2017ISBN 978 0 580 83578 0ICS 07.1
12、00.30Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2017.Amendments/corrigenda issued since publicationDate Text affectedBRITISH STANDARDBS EN ISO 6887
13、-1:2017EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 6887-1 April 2017 ICS 07.100.30 Supersedes EN ISO 6887-1:1999English Version Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules
14、for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017) Microbiologie de la chane alimentaire - Prparation des chantillons, de la suspension mre et des dilutions dcimales en vue de lexamen microbiologique - Partie 1: Rgles gnrales pour la prparation de la suspension mre
15、 et des dilutions dcimales (ISO 6887-1:2017) Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdnnungen und von Dezimalverdnnungen fr mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln fr die Herstellung von Erstverdnnungen und Dezimalverd
16、nnungen (ISO 6887-1:2017) This European Standard was approved by CEN on 14 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lis
17、ts and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under
18、 the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yu
19、goslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COM
20、IT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 6887-1:2017 EBS EN ISO 6887-1:2017EN ISO 6887-1:20
21、17 (E) 3 European foreword This document (EN ISO 6887-1:2017) has been prepared by Technical Committee ISO/TC 34 “Food products“ in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given th
22、e status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall be withdrawn at the latest by October 2017. Attention is drawn to the possibility that some of the elements of this document may be t
23、he subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 6887-1:1999. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to im
24、plement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Roman
25、ia, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 6887-1:2017 has been approved by CEN as EN ISO 6887-1:2017 without any modification. ISO 6887-1:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and
26、 definitions . 14 Principle 35 Diluents 35.1 Basic materials 35.2 Diluents for general use 35.2.1 Peptone salt solution 35.2.2 Buffered peptone water . 45.2.3 Double-strength buffered peptone water 45.3 Diluents for special purposes . 45.4 Distribution and sterilization of the diluent 45.5 Performan
27、ce testing for diluents . 56 Apparatus . 57 Sampling 68 Preparation of samples 68.1 General . 68.2 Frozen products . 78.2.1 General 78.2.2 Small samples defrosted before testing . 78.2.3 Large pieces or blocks sampled while frozen . 78.3 Hard and dry products . 88.4 Dehydrated and other low-moisture
28、 products 88.5 Liquid and non-viscous products 88.6 Acidic products . 88.7 High-fat (over 20 %) foods . 98.8 Multi-component products. 98.9 Packaged products . 98.10 Surface samples (swabs and other devices) . 109 Specific procedures.109.1 Test portion and initial suspension (primary dilution) 109.2
29、 Duration of the procedure 119.3 Pooling and compositing procedures for qualitative tests.1110 Further dilutions .1110.1 Decimal dilution series . 1110.2 Other dilution series . 12Annex A (informative) Illustrations of pooling and compositing procedures13Annex B (informative) Method for sampling fro
30、zen test pieces or blocks .18Annex C (informative) Data showing reliability of test results according to size of test portions 20Annex D (informative) Verification protocol for pooling samples for qualitative tests .23Bibliography .26 ISO 2017 All rights reserved iiiContents PageBS EN ISO 6887-1:201
31、7ISO 6887-1:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 35 Diluents 35.1 Basic materials 35.2 Diluents for general use 35.2.1 Peptone salt solution 35.2.2 Buffered peptone water . 45.2.3 Double-strength buffered peptone water 45.3 Diluent
32、s for special purposes . 45.4 Distribution and sterilization of the diluent 45.5 Performance testing for diluents . 56 Apparatus . 57 Sampling 68 Preparation of samples 68.1 General . 68.2 Frozen products . 78.2.1 General 78.2.2 Small samples defrosted before testing . 78.2.3 Large pieces or blocks
33、sampled while frozen . 78.3 Hard and dry products . 88.4 Dehydrated and other low-moisture products 88.5 Liquid and non-viscous products 88.6 Acidic products . 88.7 High-fat (over 20 %) foods . 98.8 Multi-component products. 98.9 Packaged products . 98.10 Surface samples (swabs and other devices) .
34、109 Specific procedures.109.1 Test portion and initial suspension (primary dilution) 109.2 Duration of the procedure 119.3 Pooling and compositing procedures for qualitative tests.1110 Further dilutions .1110.1 Decimal dilution series . 1110.2 Other dilution series . 12Annex A (informative) Illustra
35、tions of pooling and compositing procedures13Annex B (informative) Method for sampling frozen test pieces or blocks .18Annex C (informative) Data showing reliability of test results according to size of test portions 20Annex D (informative) Verification protocol for pooling samples for qualitative t
36、ests .23Bibliography .26 ISO 2017 All rights reserved iiiContents PageBS EN ISO 6887-1:2017ISO 6887-1:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is n
37、ormally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part i
38、n the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular
39、the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this
40、document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .i
41、so .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the
42、 World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology.This second edition cancels and replaces the first
43、 edition (ISO 6887-1:1999), which has been technically revised.A list of parts in the ISO 6887 series can be found on the ISO website.iv ISO 2017 All rights reservedBS EN ISO 6887-1:2017ISO 6887-1:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of na
44、tional standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Intern
45、ational organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those inten
46、ded for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www
47、.iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document
48、 will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and e
49、xpressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology.This second edition cancels and replaces the first edition (ISO 6887-1:1999), which has been technically revised.A list of parts in the ISO 6887 series can be found on the ISO website.
