1、BSI Standards PublicationBS ISO 12853:2015Microscopes Informationprovided to the userBS ISO 12853:2015 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 12853:2015. It supersedes BS 7012-11:1998 which is withdrawn.The UK participation in its preparation was entru
2、sted to Technical Committee CPW/172, Optics and Photonics.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The
3、British Standards Institution 2015. Published by BSI Standards Limited 2015ISBN 978 0 580 85745 4 ICS 37.020 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 S
4、eptember 2015.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS ISO 12853:2015 ISO 2015Microscopes Information provided to the userMicroscopes Informations dlivres lutilisateurINTERNATIONAL STANDARDISO12853Second edition2015-09-15Reference numberISO 12853:2015(E)BS ISO 128
5、53:2015ISO 12853:2015(E)ii ISO 2015 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2015, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including
6、photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 2
7、2 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 12853:2015ISO 12853:2015(E)Foreword iv1 Scope . 12 Normative references 13 Data provided by the manufacturer . 13.1 General information . 13.1.1 Details of origin . 13.1.2 (r) Field of application 13.1.3 (m) Imaging and illumination me
8、thods . 13.1.4 (r) Accessories . 13.2 Design information 13.2.1 Optical system 13.2.2 Stand . 23.2.3 Microscope tube . 23.2.4 Nosepiece . 33.2.5 Objectives . 43.2.6 Eyepieces 43.2.7 Condenser 53.2.8 Illuminating system . 53.2.9 Light source 53.2.10 Power supply . 5Bibliography 6 ISO 2015 All rights
9、reserved iiiContents PageBS ISO 12853:2015ISO 12853:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committe
10、es. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Inte
11、rnational Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the dif
12、ferent types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO sha
13、ll not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document i
14、s information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT)
15、 see the following URL: Foreword - Supplementary information.The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes.This second edition cancels and replaces the first edition (ISO 12853:1997), which has been technically revised
16、in order to: specify the Scope in more detail; add further examples to the field of application (see 3.1.2); update the design information (e.g. dimension of overall mechanical depth added and new Figure 1); add the optical system to the design information (see 3.2.1).iv ISO 2015 All rights reserved
17、BS ISO 12853:2015INTERNATIONAL STANDARD ISO 12853:2015(E)Microscopes Information provided to the user1 ScopeThis International Standard specifies the minimum required information to be provided to the user by the microscope manufacturer. This International Standard is not applicable to inverted micr
18、oscopes, advanced technique microscopes, and digital display microscopes.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated refere
19、nces, the latest edition of the referenced document (including any amendments) applies.ISO 8578, Microscopes Marking of objectives and eyepieces3 Data provided by the manufacturer3.1 General informationThe mandatory data, indicated (m), shall be provided by the manufacturer when the respective micro
20、scope assemblies feature the properties described. Provision of further microscope data, indicated (r), is recommended but not required.3.1.1 Details of origin3.1.1.1 (m) Manufacturer3.1.1.2 (r) Country of origin, if required3.1.2 (r) Field of applicationEXAMPLE Schools and courses, laboratories, re
21、search, industry.3.1.3 (m) Imaging and illumination methodsEXAMPLE Transmitted light, reflected light, brightfield, darkfield, phase contrast, differential interference contrast, polarization, fluorescence.3.1.4 (r) AccessoriesEXAMPLE Photographic equipment, alternative light sources, manipulators,
22、photometric equipment, image processing equipment, video equipment.3.2 Design information3.2.1 Optical system3.2.1.1 (m) Type of optical systemEXAMPLE Finite, infinite. ISO 2015 All rights reserved 1BS ISO 12853:2015ISO 12853:2015(E)3.2.2 Stand3.2.2.1 Dimension of stand, expressed in millimetres (se
23、e Figure 1):a) (m) l1, l2base dimensions (measured in x and y directions);b) (m) l3overall mechanical height (without attachments);c) (m) l5distance from exit pupil to base plane (65 mm interpupillary distance);d) (m) l7distance from exit pupil to focus drive (65 mm interpupillary distance);e) (r) l
24、4maximum height of stage surface from base plane;f) (r) l6distance from focus drive to base plane;g) (r) l8distance between the exit pupil and the objectives optical axis;h) (r) l9overall mechanical depth;i) (r) l10depth of lamp-house.3.2.2.2 (r) Mass of the stand, expressed in kilograms (including
25、body tube, but without attachments).3.2.2.3 (r) Interchangeability of components at the stand.EXAMPLE Viewing tube, stage, substage, lamp housing, nosepiece.3.2.2.4 Adjustment integrated in the stand:a) (m) coarse adjustment affecting the stage or the body tube;b) (m) operating range of coarse adjus
26、tment range, in millimetres;c) (m) fine adjustment affecting the stage or the body tube;d) (r) operating range of fine adjustment, in millimetres;e) (r) movement of fine adjustment per scale division or rotation, in micrometres;f) (r) movement of coarse adjustment per rotation, in millimetres.3.2.2.
27、5 (r) Slots integrated in the stand.3.2.2.6 (m) Tube factor q different from 1 (if tube lens is located in the stand).3.2.3 Microscope tubea) (m) Monocular, binocular, or trinocular tube; b) (m) interpupillary distance adjustment range, in millimetres;c) (m) tube factor q different from 1 (if the tu
28、be lens is located in the tube);d) (m) inclination angle of a viewing direction (see Figure 1);e) (m) inside diameter of the eyepiece tube(s) (23,2 mm or 30 mm);f) (r) dioptre adjustment;2 ISO 2015 All rights reservedBS ISO 12853:2015ISO 12853:2015(E)g) (r) tube length compensation;h) (r) focal leng
29、th of the tube lens (if the tube lens is corrected for infinite primary image distance);i) (r) splitting ratio(s) of the beam splitter(s);j) (r) distance l8between the exit pupil and the objectives optical axis (see Figure 1).Dimensions in millimetresKey1 exit pupil 6 stage2 optical axis 7 substage3
30、 viewing tube 8 base of stand4 body tube 9 focus drive5 nosepiece 10 lamp houseFigure 1 Dimensions of the stand3.2.4 Nosepiecea) (m) Number of mounting holes for objectives;b) (m) objective centration;c) (r) dimensions of screw thread other than the RMS thread. ISO 2015 All rights reserved 3BS ISO 1
31、2853:2015ISO 12853:2015(E)3.2.5 ObjectivesThe following data of the objectives, suitable for use with related microscope, shall be listed in accordance with ISO 8578:a) (m) magnification;b) (m) numerical aperture;c) (m) state of correction, field of view, and colour;d) (m) immersion medium (other th
32、an air);e) (m) tube length, in millimetres;f) (m) cover glass thickness, in millimetres;g) (m) contrast methods;h) (m) suitability for polarized-light microscopy;i) (m) iris diaphragm;j) (m) free working distance, in millimetres;k) (m) correction collar.3.2.6 EyepiecesThe following data of the objec
33、tives, suitable for use with related microscope, shall be listed in accordance with ISO 8578:a) (m) magnification;b) (m) field number;c) (m) diameter of eyepiece (23,2 mm or 30 mm);d) (r) type of correction;e) (r) interchangeability of graticules;f) (r) suitability for spectacle wearers;g) (r) diopt
34、re adjustment.4 ISO 2015 All rights reservedBS ISO 12853:2015ISO 12853:2015(E)3.2.7 Condensera) (m) Maximum numerical aperture;b) (m) suitability for contrast methods;c) (r) type of correction;d) (r) range of objectives at a given field of view;e) (r) free working distance, in millimetres;f) (r) ill
35、uminating aperture diaphragm;g) (r) filter holder;h) (r) interchangeability;i) (r) slide thickness, in millimetres.3.2.8 Illuminating system3.2.8.1 (r) Substage.EXAMPLE Interchangeability, centring possibility for the condenser, filter holder, polarizing device, illuminated field diaphragm for trans
36、mitted light.3.2.8.2 (r) Vertical illuminator for incident light.EXAMPLE Illuminating aperture diaphragm, illuminated field diaphragm, slots for accessories, polarizing device, centring possibility of diaphragms.3.2.9 Light sourcea) (m) Arrangement of the light source (incorporated in the stand or l
37、amp housing attached to the stand);b) (m) type of light source (halogen, LED, mercury arc, xenon arc, etc.);c) (m) model number;d) (m) voltage and wattage of light source;e) (m) type of current (a.c. or d.c.);f) (r) colour temperature of the light source at rated voltage;g) (r) rated lifetime of the
38、 light source.3.2.10 Power supplya) (m) Main supply voltage (V a.c.);b) (m) main supply frequency (50 Hz and/or 60 Hz);c) (m) power consumption (VA);d) (m) fuse (type rated amperage);e) (m) built-in or external;f) (m) output adjustable or fixed. ISO 2015 All rights reserved 5BS ISO 12853:2015ISO 128
39、53:2015(E)Bibliography1 ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part 1: Light microscopy6 ISO 2015 All rights reservedBS ISO 12853:2015BS ISO 12853:2015ISO 12853:2015(E) ISO 2015 All rights reservedICS 37.020Price based on 6 pagesThis page deliberately left blankBSI is
40、the national body responsible for preparing British Standards and other standards-related publications, information and services.BSI is incorporated by Royal Charter. British Standards and other standardization products are published by BSI Standards Limited.British Standards Institution (BSI)BSI Gr
41、oup Headquarters389 Chiswick High Road London W4 4AL UKAbout usWe bring together business, industry, government, consumers, innovators and others to shape their combined experience and expertise into standards -based solutions.The knowledge embodied in our standards has been carefully assembled in a
42、 dependable format and refined through our open consultation process. Organizations of all sizes and across all sectors choose standards to help them achieve their goals.Information on standardsWe can provide you with the knowledge that your organization needs to succeed. Find out more about British
43、 Standards by visiting our website at or contacting our Customer Services team or Knowledge Centre.Buying standardsYou can buy and download PDF versions of BSI publications, including British and adopted European and international standards, through our website at where hard copies can also be pur
44、chased. If you need international and foreign standards from other Standards Development Organizations, hard copies can be ordered from our Customer Services team.SubscriptionsOur range of subscription services are designed to make using standards easier for you. For further information on our subsc
45、ription products go to British Standards Online (BSOL) youll have instant access to over 55,000 British and adopted European and international standards from your desktop. Its available 24/7 and is refreshed daily so youll always be up to date. You can keep in touch with standards developments and
46、receive substantial discounts on the purchase price of standards, both in single copy and subscription format, by becoming a BSI Subscribing Member. PLUS is an updating service exclusive to BSI Subscribing Members. You will automatically receive the latest hard copy of your standards when theyre rev
47、ised or replaced. To find out more about becoming a BSI Subscribing Member and the benefits of membership, please visit a Multi-User Network Licence (MUNL) you are able to host standards publications on your intranet. Licences can cover as few or as many users as you wish. With updates supplied as
48、soon as theyre available, you can be sure your documentation is current. For further information, email .RevisionsOur British Standards and other publications are updated by amendment or revision. We continually improve the quality of our products and services to benefit your business. If you find a
49、n inaccuracy or ambiguity within a British Standard or other BSI publication please inform the Knowledge Centre.CopyrightAll the data, software and documentation set out in all British Standards and other BSI publications are the property of and copyrighted by BSI, or some person or entity that owns copyright in the information used (such as the international standardization bodies) and has formally