1、January 2013 Translation by DIN-Sprachendienst.English price group 16No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).IC
2、S 13.060.70!$*“1940761www.din.deDDIN EN ISO 6341English version EN ISO 6341:2012,English translation of DIN EN ISO 6341:2013-01Wasserbeschaffenheit Bestimmung der Hemmung der Beweglichkeit von Daphnia magna Straus (Cladocera,Crustacea) Akuter Toxizitts-Test (ISO 6341:2012);Englische Fassung EN ISO 6
3、341:2012,Englische bersetzung von DIN EN ISO 6341:2013-01Qualit de leau Dtermination de linhibition de la mobilit de Daphnia magna Straus (Cladocera,Crustacea) Essai de toxicit aigu (ISO 6341:2012);Version anglaise EN ISO 6341:2012,Traduction anglaise de DIN EN ISO 6341:2013-01SupersedesDIN EN ISO 6
4、341:1996-06www.beuth.deDocument comprises pagesIn case of doubt, the German-language original shall be considered authoritative.32Water quality Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) Acute toxicity test (ISO 6341:2012); 01.13DIN EN ISO 6341:201
5、3-01 2 A comma is used as the decimal marker. National foreword This document (EN ISO 6341:2012) has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved
6、 in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-03-05-01 AK Biotests of NA 119-01-03 AA Wasseruntersuchung. This standard has been published to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the Europea
7、n Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy*). Designation of method L 40: Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) Acute toxicity test (L 40): Method DIN EN ISO 63
8、41 L 40 The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 5667-16 DIN EN ISO 5667-16 ISO 5814 DIN EN ISO 5814 ISO 10523 DIN EN ISO 10523 ISO 15088 DIN EN ISO 15088 *) Registered in the DITR database of DIN Software GmbH, obtainable from:
9、Beuth Verlag GmbH, 10772 Berlin. DIN EN ISO 6341:2013-01 3 Expert assistance and specialized laboratories will be required to perform the analyses described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a case-
10、by-case basis as to whether and to what extent additional conditions will have to be specified. This standard has been prepared by the Normenausschuss Wasserwesen (Water Practice Standards Committee) in collaboration with the Wasserchemische Gesellschaft Fachgruppe in der Gesellschaft Deutscher Chem
11、iker (Water Chemistry Society Division of the German Chemical Society). It is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung Biologisch-kologische Gewsseruntersuchung (Gruppe M) (German standard methods for the examination of water, waste water and sludg
12、e Biological-ecological methods of analysis (group M) and describes method L 40: Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) Acute toxicity test (L 40) Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individ
13、ually or grouped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth Verlag GmbH. Standards or draft standards bearing the group title “G
14、erman standard methods for the examination of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) Sensory analysis (group B) Physical and physicochemical parameters (group C) Anions (group D) Cations (group E) Substance group anal
15、ysis (group F) Gaseous constituents (group G) Parameters characterizing effects and substances (group H) Microbiological methods (group K) Test methods using water organisms (group L) Biological-ecological methods of analysis (group M) Individual constituents (group P) Sludge and sediments (group S)
16、 Bio-assays with microorganisms (group T) Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenausschuss Wasserwesen, telephone +49 (30) 2601-2448, or from Beuth Verlag GmbH, Am DIN-Platz, Burggrafenstr. 6, 10787 Berlin. DIN
17、 EN ISO 6341:2013-01 4 Amendments This standard differs from DIN EN ISO 6341:1996-06 as follows: a) Clause 1 “Scope” has been extended to include: treated or untreated waste water, aqueous extracts and leachates, eluates of fresh water sediment, pore water of fresh water sediment; b) Clause 3 “Terms
18、 and definitions” has been included; c) the duration of the test period may be 24 h or 48 h; d) a limit test may now be performed at concentrations above 100 mg/l as long as the substance is soluble or in stable dispersion; e) Clause 6 “Reagents, test organisms and media”: requirements to meet the c
19、ulture health criteria of the Daphnia stock have been included, neonates hatched from the ephippia may also be used as test organisms, dechlorinated tap water is acceptable as culturing and dilution water, a description of the M4 medium (now acceptable as a hatching medium) has been included, the pH
20、 of the synthetic dilution water has been changed from 7,8 0,2 to 7,8 0,5, for the preparation of the stock solution for testing the reference substance, it is recommended to use a ready-made potassium dichromate solution; f) Clause 7 “Apparatus and materials”: before use, it is no longer required t
21、o wash test containers with diluted water, pipettes for sampling the test organisms have been included, g) Clause 8 “Treatment and preparation of samples”: sampling, transportation and storage of the samples should be performed as specified in ISO 5667-16, modifications and supplements related to th
22、e sample treatment: the cooled sample may be stored for 24 h (instead of 48 h), until the beginning of the test, the samples are to be stored at a temperature between 0 C and 5 C, the times and temperatures of the sample storage until the beginning of the test have been modified, information relatin
23、g to the thawing of the samples has been included, the treatment of environmental samples by way of sedimentation, centrifugation or filtration to separate residual suspended matter has been included, guidance on the aeration of the sample has been included, the test is carried out without pH adjust
24、ment of the sample, further information relating to the preparation of test solutions with poorly soluble substances has been included, information concerning test design and performance for tests with poorly soluble substances has been included, DIN EN ISO 6341:2013-01 5 information on the selectio
25、n of dilution series in the case of chemical substances and on environmental samples for test solutions has been included, information on the test batch for the determination of the LID has been included, for the definitive test on chemical substances, the separation factor for the serial dilutions
26、has been limited to 3,2; h) Clause 9 “Procedure”: the requirement for the oxygen content of the test batches has been expanded: the dissolved oxygen concentration is to be measured both in the control batches and in the test batches having the highest test concentration at the end of the test, only
27、test batches having a dissolved oxygen concentration of 2 mg/l are to be considered for the final calculations, Subclause 10.2 “Validity criteria”: the frequency of reference tests has been determined, the 24 h EC50of the potassium dichromate has been changed to be within the range 0,6 mg/l to 2,1 m
28、g/l (instead of 1,7 mg/l), limit test: it is recommended to conduct a full study if the percentage of immobilization exceeds 10 % in the limit test with 100 mg/l test substance solution; i) Clause 10 “Interpretation and validity of the results” Estimation of the EC50: if the nominal and the measured
29、 concentration of the test substance in the test medium differ significantly, it is recommended to repeat the test with a new test medium or to carry out a flow-through test; j) the expression of results now includes the LID value, if determined; k) the test report has been supplemented to include:
30、information on the treatment of environmental samples, results of the reference substance testing; l) Annex A now includes guidance for the preparation of the M4 medium; m) Annex B now includes the option to estimate the confidence levels from the graph; n) a new Annex C relating to general recommen
31、dations for stock culturing has been added; o) a new Annex D relating to the culturing of Daphnia magna for production of dormant eggs has been added; p) a new Annex F relating to the preparation of the dilution series for the determination of the LID has been added for testing waste water by means
32、of a graduated dilution, D; q) the standard has been editorially revised. Previous editions DIN EN ISO 6341: 1996-06 DIN EN ISO 6341:2013-01 6 National Annex NA (informative) Bibliography DIN EN ISO 5667-16:1999-02, Water quality Sampling Part 16: Guidance on biotesting of samples DIN EN ISO 5814, W
33、ater quality Determination of dissolved oxygen Electrochemical probe method DIN EN ISO 10523, Water quality Determination of pH DIN EN ISO 15088:2009-06, Water quality Determination of the acute toxicity of waste water to zebrafish eggs (Danio rerio) EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM
34、 EN ISO 6341 October 2012 ICS 13.060.70 Supersedes EN ISO 6341:1996English Version Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test (ISO 6341:2012) Qualit de leau - Dtermination de linhibition de la mobilit de Daphni
35、a magna Straus (Cladocera, Crustacea) - Essai de toxicit aigu (ISO 6341:2012) Wasserbeschaffenheit - Bestimmung der Hemmung der Beweglichkeit von Daphnia magna Straus (Cladocera, Crustacea) - Akuter Toxizitts-Test (ISO 6341:2012) This European Standard was approved by CEN on 14 October 2012. CEN mem
36、bers are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on applicat
37、ion to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Manage
38、ment Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
39、 Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 B
40、russels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 6341:2012: EContents Page EN ISO 6341:2012 (E) DIN EN ISO 6341:2013-01 2 Foreword 3 Introduction .4 1 Scope 5 2 Normative references 5 3 Terms and definitions .5 4 Pr
41、inciple 6 5 Test environment .6 6 Reagents, test organisms and media 7 7 Apparatus and materials .8 8 Treatment and preparation of samples .9 8.1 Special precautions for sampling, transportation, storage and treatment of water, effluent, or aqueous extract samples to be tested 9 8.2 Preparation of s
42、olutions of substances to be tested .10 9 Procedure .10 9.1 General 10 9.2 Preliminary test 11 9.3 Definitive test .11 9.4 Check of the sensitivity of the Daphnia magna and conformity with the procedure .11 9.5 Limit test .12 10 Interpretation and validity of the results .12 10.1 Estimation of the E
43、C50.12 10.2 Validity criteria .12 11 Expression of results 12 12 Test report 12 Annex A (informative) Preparation of the Elendt M4 medium 14 Annex B (informative) Example of graphical determination of the inhibition of mobility of Daphnia magna by an effluent or stock solution of a substance at a co
44、ncentration of 1 000 mg/l .17 Annex C (informative) General recommendations for stock culturing 20 Annex D (informative) Culturing of Daphnia magna for production of dormant eggs .21 Annex E (informative) Precision data 23 Annex F (informative) Dilution level D Preparation of the dilution series for
45、 the determination of the LID .24 Bibliography 26 Foreword This document (EN ISO 6341:2012) has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall
46、be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by April 2013, and conflicting national standards shall be withdrawn at the latest by April 2013. Attention is drawn to the possibility that some of the elements of this document m
47、ay be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 6341:1996. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound
48、to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
49、Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 6341:2012 has been approved by CEN as a EN ISO 6341:2012 without any modification. EN ISO 6341:2012 (E) DIN EN ISO 6341:2013-01 3 IntroductionThis International Standard specifies a procedure for the determination of the acute toxicity of chemicals, waters and waste waters to the water flea Daphnia magna Straus.The evaluation o
