1、BRITISH STANDARD BS5218:1989 EN21:1988 Wood preservatives Determination of the toxic values against Anobium punctatum (De Geer) by larval transfer(laboratory method) This European Standard EN21 has the status of a British Standard. UDC 674.048.4:620.193.87BS5218:1989 This British Standard, having be
2、en prepared under the directionof the Wood PreservationStandards Committee, was published underthe authority of theBoardofBSI andcomesintoeffect on 28April1989 BSI 12-1999 First published June1975 First revision April1989 The following BSI references relate to the work on this standard: Committee re
3、ference WPC/10 Draft for comment 85/52675 DC ISBN 0 580 17087 X Cooperating organizations The European Committee for Standardization, under whose supervision this European Standard was prepared, comprises the national standards organizations of the following Western European countries. Austria Oeste
4、rreichisches Normungsinstitut Belgium Institut belge de normalisation Denmark Dansk Standardiseringsraad Finland Suomen Standardisoimisliito, r.y. France Association franaise de normalisation Germany Deutsches Institut fr Normung e.V. Greece Hellenic Organization for Standardization Iceland Icelandi
5、c Council for Standardization Ireland National Standards Authority of Ireland Italy Ente Nazionale Italiano di Unificazione Luxemburg Inspection des travails et des mines Netherlands Nederlands Normalisatie-instituut Norway Norges Standardiseringsforbund Portugal Instituto Portugus de Qualidade Spai
6、n Instituto Espaol de Normalizacin Sweden Standardiseringskommissionen i Sverige Switzerland Association suisse de normalisation UnitedKingdom British Standards Institution Amendments issued since publication Amd. No. Date of issue CommentsBS5218:1989 BSI 12-1999 i Contents Page Cooperating organiza
7、tions Inside front cover National foreword ii Brief history 2 Text of EN21 3 National appendix A Inside back cover National appendix B Inside back cover National appendix C Inside back coverBS5218:1989 ii BSI 12-1999 National foreword This British Standard has been published under the direction of t
8、he Wood Preservation Standards Committee. BS5218 was first published in1975. This revision supersedes the1975 edition which is withdrawn. BS5218 is identical with EN21:1988 “Wood preservatives. Determination of the toxic values against Anobium punctatum(De Geer) by larval transfer(Laboratory method)
9、 published by the European Committee for Standardization(CEN). EN21:1988 was prepared as a result of discussion in CEN Technical Committee38 “Methods of test of wood preservatives”, in which the UK participated. National appendix A gives the constitution of the committee responsible for the UK par
10、ticipation in the preparation of this standard. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obliga
11、tions. Summary of pages This document comprises a front cover, an inside front cover, pagesi andii, theEN title page, pages2 to14, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendm
12、ent table on the inside front cover.EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN21 November1988 UDC 674.048.4:620.193.87 Supersedes EN21, September1974 Key words: Wood, wood preservatives, pesticides, insecticides, protection against the pest, laboratory tests, determination, effectiveness l
13、imit, Anobiidae. English version Wood preservatives; Determination of the toxic values against Anobium punctatum (De Geer) by larval transfer (laboratory method) Produits de prservation des bois; Dtermination du seuil defficacit contre Anobium punctatum (De Geer) par transfert de larves(Mthode de la
14、boratoire) Holzschutzmittel; Bestimmung des Giftwerkes gegenber Anobium punctatum (De Geer) durch Umsetzen von Larven(Laboratoriumsverfahren) This European Standard was accepted by CEN on4 December1987. CEN members are bound to comply with the requirements of the CEN/CENELEC Rules which stipulate th
15、e conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Central Secretariat or to any CEN member. This European Standard exist
16、s in three official versions(English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to CEN Central Secretariat has the same status as the official versions. CEN members are the national standards organ
17、izations of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxemburg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and UnitedKingdom. CEN European Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central S
18、ecretariat: rue de Stassart 36, B-1050 Brussels CEN1988 Copyright reserved to all CEN Members. Ref. No. EN21:1988 EEN21:1988 BSI 12-1999 2 Brief history This European Standard was drawn up by the Technical Committee CEN/TC38 “Methods of test for wood preservatives”, the Secretariat of which is held
19、by AFNOR. According to the Common CEN/CENELEC Rules, the following countries are bound to implement this European Standard: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxemburg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and UnitedKingdom. Co
20、ntents Page Brief history 2 0 Introduction 3 1 Object 3 2 Field of application 3 3 Principle 3 4 Test materials 3 5 Sample of the preservative 5 6 Test specimens 5 7 Procedure 6 8 Expression of results 8 9 Test report 8 Annex A Example of a test report 10 Annex B Precautions against mite infestation
21、 12 Annex C Culturing technique for Anobiumpunctatum 12 Figure 1 Pattern and shape of holes 8 Table 1 Number of larvae and test specimens 4 Table 2 Diameter of holes 7 Table 3 Results 11EN21:1988 BSI 12-1999 3 0 Introduction This European Standard describes a laboratory method of test which gives a
22、basis for the assessment of the effectiveness of a wood preservative against Anobium punctatum. It allows the determination of the concentration at which the product completely prevents the survival of Anobium punctatum larvae in impregnated wood of a susceptible species. Toxic values determined by
23、larval transfer appear to be somewhat higher than those derived from the test of egg-laying and larval survival 1)and therefore give a safety margin. Although an infestation normally starts from egg-laying, a larval transfer test is applicable when considering the situation of treated wood being put
24、 in contact, during repair work, with wood that might be infested. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further recommended tha
25、t results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When using products which are very active at very low concentration, it is of great importance that suitable precautions be taken to isolate and separate as f
26、ar as possible operations involving chemical products, other products, treated wood, all clothing and laboratory apparatus. Suitable precautions shall include the use of separate rooms, areas within the rooms, extraction facilities, conditioning chambers and special personnel training. 1 Object This
27、 European Standard specifies a method for the determination of the toxic values of a wood preservative against larvae of Anobium punctatum (de Geer), introduced into wood which has been treated previously by full impregnation. 2 Field of application This method is applicable to: water-insoluble chem
28、icals which are being studied as active insecticides; organic water-insoluble formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; water-soluble materia
29、ls, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 3 Principle Impregnation of several sets of test specimens of susceptible wood with solutions in which the concentrations of preservatives are ranged in a given pro
30、gression. Introduction of Anobium punctatum larvae into these specimens, and determination of their survival rate. Comparison of the results at the different concentrations and, taking into account the survival in the untreated and solvent or diluent treated control specimens, derivation of the toxi
31、c values of the product under test. 4 Test materials 4.1 Biological material Anobium punctatum (de Geer) larvae 4.1.1 Origin of larvae. Obtain the larvae from cultures reared as described inAnnex C. Cut up this wood and extract the larvae in an area separate from the test environments(see4.3.1 to4.3
32、4), so as to avoid the risk of introducing mites. 1) EN49.EN21:1988 4 BSI 12-1999 Prepare the storage blocks from Scots pine sapwood, of dimensions50mm 25mm 15mm each with10 evenly spaced holes(see7.2) drilled into one of the wide faces with the drill(see4.3.10). Before inserting the larvae into th
33、e storage blocks, keep them overnight in small glass receptacles. Then sort the larvae into small, medium and large sizes. The large larvae, with a mass greater than5mg, are not used for this test 2) . Examine all the other larvae under a binocular microscope and destroy those that are damaged or in
34、fested with mites, keeping only those that are in perfect condition. Insert the small and medium larvae into separate sets of storage blocks, placing each larva head first into a drilled hole. Keep these filled storage blocks holes uppermost, in the test containers(4.3.11) closed with filter paper f
35、ixed with emulsion adhesive. Keep the larvae in the storage blocks in the culturing chamber(see4.3.1) for not less than two months before using them in a test. 4.1.2 Provision of larvae. Carefully cut out the larvae from the storage blocks and examine them under a binocular microscope. Destroy any l
36、arvae that show injury or mite infestation, or that do not respond by movement when touched. It is particularly important to avoid including mite-infested larvae (seeAnnex B). Keep those larvae that are between2mg and5mg 2)in mass and in perfect condition overnight, separate from one another, in cle
37、an receptacles in the culturing chamber(see4.3.1). Then re-examine them, and again reject any which do not show normal movements. 4.1.3 Choice of larvae. The larvae used in the test shall be between2mg and5mg in mass, and the10 larvae placed in each test specimen shall have a mean mass of approximat
38、ely3.5mg 3) . The numbers of larvae required are indicated in the following table. Table 1 Number of larvae and test specimens 4.2 Products and reagents 4.2.1 Solvents and diluents for water-soluble preservatives: distilled or deionized water. for preservatives that will be dissolved in or diluted w
39、ith organic solvents: a suitable volatile liquid 4) . This shall not leave a residue in the wood at the end of the post-treatment conditioning period which would have a toxic effect on the insects. 4.2.2 Xylene 2) Experienced operators can judge the sizes well enough by eye. 3) The mass may be judge
40、d by eye by comparison with larvae of known mass. Type of test specimen Concentration of preservative Number of test specimens Number of larvae Without radiography available With radiography available Without radiography available With radiography available Treated specimens Treated specimens Treate
41、d specimens Treated specimens Treated specimens etc. 1 2 3 4 5 10 10 10 10 10 5 5 5 5 5 100 100 100 100 100 50 50 50 50 50 Untreated control specimens 0 10 5 100 50 Solvent or diluent treated control specimens(including water) 0 5 5 50 50 Total for five concentrations 650 350 4) Do not use benzene a
42、s a solvent because it poses a health risk to those conducting the test.EN21:1988 BSI 12-1999 5 4.3 Apparatus 4.3.1 Culturing chamber (incubator or room), with air circulation, and controlled at between20 C and22 C with a tolerance of 1 C, and at between80%r.h.and85%r.h.with a tolerance of5%r.h. 4.3
43、2 Conditioning chamber, well ventilated and controlled at20 2 C and at655%r.h. 5) 4.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out 6) . 4.3.4 Testing chamber (incubator or room), with conditions identical to those of the culturing chamber(see4.3.1).
44、4.3.5 Treatment vessels (beakers), of material that does not react with the preservative under test, for example of glass for organic products and of plastics materials for salts containing fluorine. 4.3.6 Weights, chemically inert, for ballasting the test blocks. 4.3.7 Protective gloves 4.3.8 Vacuu
45、m vessels, fitted with stopcocks. 4.3.9 Vacuum pump, fitted with a pressure gauge, and capable of maintaining a pressure of700Pa 7) . 4.3.10 Drill, provided with a bit capable of drilling cylindrical or conical holes, as specified in7.2. 4.3.11 Test containers (jars), with rests, suitable for holdin
46、g the test specimens. 4.3.12 Ordinary laboratory equipment including two analytical balances. 4.3.13 X-ray apparatus (if desired), with tungsten target and beryllium window, with voltage and current continuously variable in the ranges: 5 Sample of the preservative The sample shall be representative
47、of the product under test. 6 Test specimens 6.1 Species of wood The reference species is Scots pine, Pinus sylvestris Linnaeus. Additional tests may be made using other species but, if so, this shall be stated in the test report. 6.2 Quality of wood Use only sound sapwood, straight-grained, without
48、knots and with a low resin content. Cut the specimens from wood of average growth rate(2.5to8annual rings per centimetre). The proportion of summer wood in the annual rings shall not exceed about30%of the whole. Take the test blocks from trees felled during their dormant period(generally from Octobe
49、r to May in temperate climates) and converted within4 weeks of felling. They may await conversion for longer periods in cold climates, e.g.Scandinavia. The wood shall have been neither floated nor subjected to any chemical or heat treatment 8) . It shall be air-dried and shall not have been stored for more than5years. 6.3 Provision of test specimens Cut the specimens from planed strips having a cross section25mm 15mm and with the wide longitudinal faces orientated tangentially. The individual specimens shall be cut
