GOST 32293-2013 Testing of chemicals of environmental hazard Alga and Cyanobacteria Growth Inhibition Test《环境危险化学品的试验 藻类和蓝藻 生长抑制试验》.pdf

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1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32293 2013 , (OECD, Test No. 201, IDT) 322932013 II , 1.0 92 . 1.2 2009 . , . , , , . 1 - , , ( ) 2 3 , ( 5 2013 . 61) : ( 3166) 004-97 ( 3166) 004-97 AM BY KG MD RU UA - 4 22 2013 . 777- 322932013 1 2014 . 5 OECD Test N

2、o. 201:2011 Alga, Growth Inhibition Test ( ). (en). (IDT) 6 , . () . , , 2015 , 322932013 1 , Testing of chemicals of environmental hazard Alga and Cyanobacteria, Growth Inhibition Test 20140801 1 () . 2 : 14442:2006 . , , (Water quality - Guidelines for algal growth inhibition tests with poorly sol

3、uble materials, volatile compounds, metals and waste water); 5667-16:1998 . . 16. (Water quality - Sampling - Part 16: Guidance on biotesting of samples); 8692:2012 . (Water quality Fresh water algal growth inhibition test with unicellular green algae). 3 : 3.1 (Biomass): , , , , 1 . , , , . -. 3.2

4、Lowest Observed Effect Concentration LOEC): , () ( p 0,05) . , LOEC, , LOEC. , , LOEC. 3.3 (No Observed Effect Concentration NOEC): , LOEC, () . 3.4 () (Coefficient of variation): , . . () () . 322932013 2 3.5 ECx: , %- (, 50%-) () ( , ). EC, , ErC, EyC. 3.6 (Response variable): , . , -. 3.7 (Growt

5、h medium): , () . , . 3.8 (Yield): , . 3.9 ( ) (Growth rate): . 3.10 (Specific growth rate): , () . 4 () . () 72 . () . () , , . ( ) () , . 5 5.1 () . 1 . , , ( , , ). 5.2 , ( ) . , , , %- (, 50%-) ErCx(, ErC50). 5.3 , . , , , %- (, 50%-), EyCx(, EyC50). 5.4 (LOEC) (NOEC). 322932013 36 6.1 , - Pow .

6、 . 6.2 : - ; - ; - ; - ; - ; - pKa; - , . 7 : - 16 72 , , 0,92 24 (-1). ( ). , , , , . , 16- () . () . 48 () , 16; - ( 0 1, 1 2 2 3- 72 ) 35%. , ; - 7% Pseudokirchneriella subcapitata Desmodesmus subspicatus. 10%. 8 , 3,5-. . . 9 , , . , , , , , , (, , ) 1 3. 322932013 4 10 10.1 10.1.1 , . . () . 10.1.2 , , , (CO2) . . 10.1.3 : - : , 2; - : , (), . 4 , , 2 , ; - : . . - , , . .

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