1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 15522 KS I ISO 15522 :2010 2010 8 2 http:/www.kats.go.krKS I ISO 15522:2010 : ( ) ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：2001 1 6 ：2010 8 2 2010-0302 ： ： ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 15522:2010 i .1 1 1 2 1 3 .1 4 2
2、 5 3 6 3 6.1 3 6.2 3 6.3 () 4 6.4 .4 6.5 .4 6.6 4 6.7 .4 7 4 8 5 8.1 5 8.2 .5 8.3 .5 8.4 UV/ 5 8.5 pH .5 9 5 9.1 .5 9.2 5 9.3 (preculture).6 9.4 (biomass) 6 9.5 .6 9.6 .6 10 .7 10.1 7 10.2 .7 11 .8 12 8 13 .8 A() 9 A.1 .9 A.2 .9 B() 3.5-dichlorophenol 10 C() 11 12 KS I ISO 15522:2010 Water qualityDe
3、termination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms 1999 1 ISO 15522, Water qualityDetermination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms . 1 . . 6 . , . , . 1 , . 2 , / (9.4 ). 3 (respirom
4、etric) (KS I ISO 8192) . 2 . . ( ) . KS I ISO 566716, 16： KS I ISO 8192, KS I ISO 11733, 3 KS I ISO 15522:2010 2 . 3.1 (growth) , . . 530 nm (OD) , (OD 530 ) . 3.2 (growth curve) . 3.3 (specific growth rate) (t) (x) 1/xdx/dt (h 1 ) . 3.4 (growth inhibition) . . 3.5 (inhibition curve) . 3.6 (effectiv
5、e concentration) EC , 50 %(EC 50 ), 20 %(EC 20 ), 80 %(EC 80 ) 4 , (222) . 4.5 6 . . 530 nm , (OD 530 ) . % . (semilogarithmic curve) EC . (6.7 ). KS I ISO 15522:2010 3 5 , (222) . 6 . 6.1 6.2 6.2.1 6.2.1.1 A anhydrous potassium dihydrogenphosphate(KH 2 PO 4 ) 8.5 g anhydrous dipotassium hydrogenpho
6、sphate(K 2 HPO 4 ) 21.75 g disodium hydrogenphosphate dihydrate(Na 2 HPO 4 2H 2 O) 33.4 g ammonium chloride(NH 4 Cl) 0.5 g (6.1) 1 000 mL pH 7.4 . . 6.2.1.2 B 1 000 mL (6.1) magnesium sulfate heptahydrate(MgSO 4 7H 2 O) 22.5 g . 6.2.1.3 C 1 000 mL (6.1) calcium chloride dihydrate(CaCl 2 H 2 O) 36.4
7、g . 6.2.1.4 D 1 000 mL (6.1) iron(III) chloride hexahydrate(FeCl 3 6H 2 O) 0.25 g . . (HCl) . 6.2.1.5 E . boric acid(H 3 BO 3 ) 50 mg cobalt chloride hexahydrate(CoCl 2 6H 2 O) 50 mg manganese sulfate monohydrate(MnSO 4 H 2 O) 15 mg disodium molybdate dihydrate(Na 2 MoO 4 2H 2 O) 15 mg nickel chlori
8、de hexahydrate(NiCl 2 6H 2 O) 10 mg KS I ISO 15522:2010 4 zinc sulfate heptahydrate(ZnSO 4 7H 2 O) 50 mg 1 000 mL . 6.2.1.6 F (organic substrate) ( ) 80 g 60 g (sodium acetate) 1 000 mL (6.1) KS I ISO 11733 . 6.2.2 1 000 mL 800 mL (6.1) . 10 mL A 1 mL B , E (E ). 25 mL F (6.1) 1 000 mL . 6.3 () 1 00
9、0 mL (6.1) sodium azide(NaN 3 ) ： . 6.4 1 000 mL (6.1) (NaOH) 40 g ： pH . 6.5 1 000 mL (6.1) (H 2 SO 4 ) 98 g ： pH . 6.6 . . (stock solution) 1 000 mL 1 000 mg . pH , 6.4 6.5 pH (7.00.2) . pH . KS I ISO 566716 . . KS I ISO 8192 . 6.7 1 000 mL 3.5-dichlorophenol 1 000 mg , 6.4 6.5 pH (7.00.2) . 7 . .
10、 KS I ISO 15522:2010 5 . 15 , . . , 4 24 . 8 . . 8.1 1 000 mL 100 mL, . 8.2 150 r/min . 8.3 (222) 8.4 UV/ 1 cm 4 cm 8.5 pH 9 9.1 ( A ). . a) 1 mg/L, 10 mg/L, 100 mg/L (controls) . b) 5 . c) 1 , 100 mg/L . 9.2 100 mL . (6.6) F T (6.7) F R F B / , KS I ISO 15522:2010 6 F C . . . KS I ISO 566716 . 9.3
11、(preculture) 1620 . (8.1) (6.2) 20 mL 0.5 mL (7) . . , (5) 150 r/min 1620 . (9.4) . 1 cm 0.6 4 cm 1.5 , (logarithmic phase) . 9.4 (biomass) 530 nm (, ) , . (OD 530 ) . 1 cm (cuvettes) 1 mL , 4 cm 5 mL . 20 mL (6.3) (vial) . 24 . (6.2) 20 mL F C (9.2) . 1 KS I ISO 7027 . formazine nephelometric units
12、(FNU) . . 2 . , . 9.5 1 000 mL (6.2) ( 400 mL .). (9.3) 1 000 mL 3 mL , . . 1 3 1 cm (0.10.15) OD 530 . 9.6 . 9.6 20 mL , , (9.2 A ) . 25 mL (9.3) . 4 cm , . . ( , ), . KS I ISO 15522:2010 7 6 . ( 4.5). . . . (logarithmic phase) , . , . 10 10.1 . , (9.2), ( OD 530 ) . . B . 10.2 I . I a c t c B B B
13、B 100 B c ： F B (OD 530 ) B t ： F T (OD 530 ) B a ： (F T ) (OD 530 ) log % . . EC 50 50 % . EC 50 95 % EC . EC 20 , EC 80 . F R EC . , . EC 50 100 mg/L (EC 20 ) (100 mg/L) (LID) KS I ISO 15522:2010 8 C . 11 , . (EC 20 ) . . , . . EC 50 3.5-dichlorophenol EC 50 4 12 mg/L . . F B (9.2) 1 cm 0.8 OD 530
14、 (9.4) . , . 12 23 1995 . 3.5- dichlorophenol potassium cyanide 1 . . 1 EC 20 (mg/L) 29 4.2 1.5 36 % EC 50 (mg/L) 33 8.1 2.4 29 % 3.5-dichlorophenol EC 80 (mg/L) 29 15.9 5.2 33 % EC 20 (mg/L) 18 5.5 3.4 62 % EC 50 (mg/L) 17 12.3 5.3 43 % potassium cyanide EC 80 (mg/L) 13 27.7 13.6 49 % (h 1 ) 69 0.5
15、6 0.19 34 % (OD 530 ) 69 1.04 0.47 45 % 13 . a) (KS I ISO 15522) b) c) d) pH e) f) EC 50 g) , , , EC 50 EC 20 EC 80, h) KS I ISO 15522:2010 9 A () A.1 / (mg/L) / a(mL) (9.5)(mL) (9.5)(mL) (6.1) (mL) (mL) 1 F T2 F T 1 0.025 20 0 5 25 3 F T4 F T 10 0.25 20 0 4.75 25 5 F T6 F T 100 2.5 20 0 2.5 25 7 F
16、R8 F R 5 0.125 20 0 5 25 9 F B10 F B 0 0 20 0 5 25 11 F C/ 1 0.025 0 20 5 25 12 F C/ 10 0.25 0 20 4.75 25 13 F C/ 100 2.5 0 20 2.5 25 a 1 g/L(6.6 6.7 ). . A.2 F T F C . 2mg/L, 4mg/L, 8mg/L, 16mg/L, 32mg/L KS I ISO 15522:2010 10 B () 3.5-dichlorophenol B.1 3.5-dichlorophenol (h) KS I ISO 15522:2010 1
17、1 C () (D) , 20 % (test batch) “ (LID)” . , 1/4 (25 % ) D 4 . 6.2.2 6.2.1.1 6.2.1.5 . (6.2.2 6.2.1.6 ) . LID 2 10 mL(9.6 ) . . D 2 . 25 mL C.1 . C.1 (/ ) mL mL 1/2 2 12.5 12.5 1/3 3 8.3 16.7 1/4 4 6.2 18.8 1/6 6 4.2 20.8 1/8 8 3.1 21.9 1/12 12 2.1 22.9 1/16 16 1.4 23.6 1/24 24 0.9 24.1 1/32 32 0.8 2
18、4.2 0 0 25 KS I ISO 15522:2010 12 1 KS I ISO 7027, 2 Methods for the Examination of Waters and Associated Materials. Determination of the Toxicity of Substances to Aerobic Bacteria by Measurement of Growth inhibition. 1985-6. HMSO. ISBN 0-11- 752037-3 3 ALSOP, G. M., Waggy, G.T. and Conway, R. A. Ba
19、cterial growth inhibition test. J. Water Pollut. Control, 52(10), 1980, pp. 2452-2456 4 Strotmann, U. J., Eglsaer, H. and Pagga, U. Development and evaluation of a growth inhibition test with sewage bacteria for assessing bacterial toxicity of chemical compounds. Chemosphere, 28, 1994, pp. 755-766 5
20、 Strotmann, U. J. and Pagga, U. A growth inhibition test with sewage bacteriaResults of an international ring test 1995. Chemosphere, 32, 1996, pp. 921-933 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KS I ISO 15522 :2010 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water quality Determination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms ICS 13.060.70 Korean Agency for Technology and Standards http:/www.kats.go.kr
