1、 ISO 2014 Microscopes Values, tolerances and symbols for magnification Microscopes Valeurs, tolrances et symboles de grossissement INTERNATIONAL STANDARD ISO 8039 Third edition 2014-12-01 Reference number ISO 8039:2014(E) ISO 8039:2014(E)ii ISO 2014 All rights reserved COPYRIGHT PROTECTED DOCUMENT I
2、SO 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be r
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4、rd iv 1 Scope . 1 2 Normative references 1 3 Terms and definitions . 1 4 Symbols for magnification of imaging components . 1 5 Values and tolerances for magnification . 1 5.1 Values for magnification . 1 5.2 Tolerance of values of magnification for imaging components 1 Bibliography 3 ISO 2014 All ri
5、ghts reserved iii ISO 8039:2014(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body in
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11、 URL: Foreword Supplementary information. The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes. This third edition cancels and replaces the second edition (ISO 8039:2012), of which it constitutes a minor revision.iv ISO 2014 A
12、ll rights reserved Microscopes Values, tolerances and symbols for magnification 1 Scope This International Standard specifies a series of values, tolerances, and symbols for the magnification of imaging components of light microscopes and defines a number of imaging components and magnifying systems
13、 to which they apply. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced documen
14、t (including any amendments) applies. ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part 1: Light microscopy 3 Terms and definitions For the purposes of this document, the terms and definitions given in ISO 10934-1 apply. 4 Symbols for magnification of imaging components Tabl
15、e 1 gives the symbols that shall be used when referring to the magnification of imaging components and combinations thereof and gives examples of methods of expression. 5 Values and tolerances for magnification 5.1 Values for magnification Values for the magnification of imaging components or magnif
16、ying systems should be one of the values given in Table 2. The products or quotients of any two values in the table are also to be considered as values within the table. The table can be extended by a factor of 10 per row. 5.2 Tolerance of values of magnification for imaging components Tolerances of
17、 values of magnification shall be as given in Table 3. INTERNATIONAL ST ANDARD ISO 8039:2014(E) ISO 2014 All rights reserved 1 ISO 8039:2014(E)Table 1 Symbols for magnification and methods of expression Component Symbol Methods of expression Preferred Alternative Objective: a) corrected for finite p
18、rimary image distance b) corrected for infinite primary image distance M OM O M O= 25 : 1 M O= 25 25 : 1 or 25 25 Eyepiece M E M E= 10 10 Tube lens q q = 1,25 1,25 Projection lens M PHOT M PHOT= 2,5 : 1 2,5 : 1 Total (lateral) magnification of microscope: a) for visual observation b) for real image
19、M TOT VISM TOT PROJ M TOT VIS= 500 M TOT PROJ= 500 : 1 500 500 : 1 Table 2 Values for magnification .0,32 0,4 0,5 0,63 0,8 1 1,25 1,6 2 2,5 3,2 4 5 6,3 8 10 12,5 16 20 25 32 40 50 63 80 100 125 160 200 250 320 400 500 630 800 1 000 1 250 1 600 2 000. NOTE 1 The values have been taken from the R10 se
20、ries in ISO 3:1973. NOTE 2 The value 0,32 has been rounded from its R10 series value. NOTE 3 Besides the values in this table, the following values are also in use: 1,5 15 30 60 150. Table 3 Tolerances on magnification System/component Tolerance % Objective 5 Tube lens 2 Projection lens 2 Eyepiece 5
21、2 ISO 2014 All rights reserved ISO 8039:2014(E) Bibliography 1 ISO 3:1973, Preferred numbers Series of preferred numbers 2 ISO 10934-2, Optics and optical instruments Vocabulary for microscopy Part 2: Advanced techniques in light microscopy ISO 2014 All rights reserved 3 ISO 8039:2014(E) ISO 2014 All rights reserved ICS 37.020 Price based on 3 pages
