1、Designation: E961 97 (Reapproved 2013)Standard Specification forBlood Sedimentation Tube, Wintrobe, Glass, Reusable1This standard is issued under the fixed designation E961; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year
2、of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers reusable blood sedimentationtubes suitable for determining sedimentation rates and thevol
3、ume of packed red blood cells.1.2 The values stated in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.1.3 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of t
4、his standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2E438 Specification for Glasses in Laboratory ApparatusE920 Specification for Commercially Packaged LaboratoryApparatus
5、E921 Specification for Export Packaged Laboratory Appa-ratusE1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United States Government Procure-mentsE1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3. Terminology3.1 Definitions of Terms Specific t
6、o This Standard:3.1.1 reusableCapable of being used again.3.1.2 WintrobeThe surname of the individual responsiblefor the design of the Wintrobe tube and the method of use.34. Classification4.1 This specification covers a tube that is intended to beused until it is no longer considered a functional d
7、evice for thepurpose intended.5. Materials5.1 GlassThe tubes made to this specification shall befabricated from borosilicate glass, Type I, Class B, or soda-lime glass, Type II, in accordance with Specification E438.6. Dimensions and Graduations6.1 DimensionsThe tube shall be made of tubing with ano
8、utside diameter (O.D.) of 7.0 to 8.0 mm with an insidediameter (I.D.) of 2.9 to 3.3 mm. The uniformity of the boreshall be 60.1 mm throughout the tube. The tube shall be 110to 117 mm long and have a graduated scale of 105 6 0.25 mmfrom the inside bottom of the tube. The tube shall be legiblymarked w
9、ith the manufacturers or vendors name or mark andpossess a frosted area for marking purposes.6.2 Graduation ScaleThe tube shall be graduated 105 60.25 mm in 1-mm divisions and numbered every 1 cm with twosets of numerals. One set of graduation numerals shall be from0 to 9 cm down the left side of th
10、e graduation scale and theother set of g (20 to 25C) for 15 min. Remove tube from thesolution and thoroughly rinse in tap water followed by distilledwater. Dry the tube by rubbing vigorously, 5 to 10 strokes, witha laboratory cloth or tissue. This appearance of the markingsshould be the same as befo
11、re the test, when judged by eyeunder normal room lighting.6.3 Resistance to Centrifugal Force TestFill the tube withwater to the top graduation line and place in a centrifuge. Thespeed and dimensions of centrifuge headshall be such that theinside bottom of the tube is subjected to arelative centrifu
12、galforce of (RCF) not less than 2 500 gravities. Calculate therelative centrifugal force as follows:RCF = relative centrifugal force in gravities,RCF = 00001118 r N2gravities,r = rotating radius to inside or outside of tube in centimetre,andN = rotating speed in revolutions per minute.6.4 For additi
13、onal sampling and testing data, see Specifica-tion E1157.1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatusand is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Nov. 1, 2013. Published December 2013. Originallyapproved in
14、 1983. Last previous edition approved in 2008 as E961 97 (2008).DOI: 10.1520/E0961-97R13.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summ
15、ary page onthe ASTM website.3Wintrobe, Maxwell M., “Laboratory Evaluation of Erythrocytes,” ClinicalHematology, Seventh Ed., 1974, pp. 109 134.Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States17. Packaging7.1 For packaging, select from
16、either Specification E920,Specification E921, or Practice E1133.8. Keywords8.1 blood; glass; reusable; sedimentation rate; wintrobeASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard
17、are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif no
18、t revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which
19、you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,Unit
20、ed States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).E961 97 (2013)2
