GOST 31982-2012 Food products feeds food raw materials Method of determination of b-agonists by Gas chromatography Mass spectrometry《食品 饲料 食品原料 利用气相色谱法的b-兴奋剂测定方法 质谱法》.pdf

1、 , , - - , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)319822012, - 1.092 . 1.22009 . -, . , , 1 ( ), - ( )2 3 , (- 3 2012 . 54-) : ( 3166) 00497 ( 3166) 00497 BYKZKGRUTJUZ 4 27 2013 . 241- 1 2014 .5 5403220106 - , . () - . , - , 2013 -, II 3198220121 .12 13 .24 .25 , ,

2、 , .26 47 .88 109 - 1110 .1311 1312 .1413 1415III 319822012 , , - - Food products, feeds, food raw materials. Method of determination of c98-agonists by Gas chromatography.Mass spectrometry1 , , - ( -) -. 0,1 100,0 /. - , .2 : 12.1.00588 . - 12.1.00776 . . 12.1.01893 . - . 12.1.01979 . . - 12.2.0852

3、002 , . . - 6175 . . 177074 ( 104283, 478880) ., , , . 311877 . . 419875 . . 423377 . . 432877 . . 699577 . -. 929374 . 980584 . 13496.080 , . 1386768 . 1 319822012 20140701 2230076 . . 241042001 . 2414780 . 2436380 . . 2533682 . , - , 1 , - . - (), () . , , , , .3 - - - -. - - , .4 - :- 1030;- .841

4、06;- .(220 c177 20) ;- .4951;- 30%80%. , .5 , , , 5.1 - , :- -, 45 650 - (. . .), 1,0 . . . : 2 ( 45 350 . . . 1 ) / m/z 284 10/1;- 30 c180 0,25 c180 0,25 30;- - ;- ;- 10100 3, 40200 3, 2001000 3,1532%(TransferpettorBrand,)*;* . .2 319822012- IIId=0,01,- e=100 d 24104;- 250 ;- - ;- -;- 20 280 / - 30

5、 100 ;- 3500 / 10 25 , 15,50 3 1,5 3;- 6 3, - 18 50 ;- ;- 4 3 ;- 40 3 ;- 2 3 , 100 3;- 2-10-2, 2-100-2, 2-1000-2 1770;- -2-100-32 25336;- 20 1 3;- , 5 ;- 1000 /;- pH- c1770,01 . ;- 0,45 ;- - 25 300 .5.2 :- () , 6.0;- 9293;- Subtilisine A (Sigma, , P-5380)*;- 61, . .;- 3118, . .;- 65 %-, . .;- , . .;

6、- 24147, . . .;- , . .;- N-N- () (Sigma, , -7891)*;- () (Aldrich, , 19,552-9)*;- (), . .;- N,N- () (Sigma, , T-5634)*;- () (Sigma, , T-4252)*;- - ;-.;- 24363, . .;- 4328, . .;- - 6995;- , . .;- Helix pomatia (Merck, , 1.04114)*;- ;- 9805, . . .;- 22300, . .;- Bondesil C18 40 (Varian, , 12213012)*;*

7、. .3 319822012- 20 (Sigma, , P-2287)*;- 4233, . .;- 4198;- , . .;- ()-, . . 2 . . 3 . . 13867.5.3 - - :- d9 98,0 %(RIKILT*, WageningenUR)*;- 98,0 %(RIKILT, Wageningen UR)*;- d6 98,0 %(RIKILT, WageningenUR)*;- 99,0 %(Boehringer, Ingelheim)*;- d6 98,0 %(RIKILT, WageningenUR)*;- 98,0 %(SIGMA, Deisenhof

8、en)*;- 99,0 %(RIKILT, Wageningen UR)*;- d11 98,0 %(RIKILT, WageningenUR)*;- 99,0 %(SIGMA, Deisenhofen)*;- d9 98,0 %(RIKILT, WageningenUR)*;- :1) (IRMM*4, BCR-504)*;2) , (IRMM, BCR-648)*;3) (IRMM, BCR-649)*. , - - , .6 6.1 6.1.1 - =0,2 /3pH8,0 1000 3 24,2 ()- 14,7 , 800 3 , , (8,0 c177 0,1) =1/3(6.3.13) . 2 4 1 .6.1.2 =0,1 /35,0 1000 3 13,6 , 900 3 , , (5,0 c177 0,1) =1/3(6.3.13) . 2 4 3 .* . .*