GOST 32290-2013 Testing of chemicals of environmental hazard Activated sludge respiration inhibition test (Carbon and Ammonium Oxidation)《环境危险化学品的试验 活性污泥 呼吸抑制试验 (碳和铵氧化)》.pdf

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1、 , ( )INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC) 32290 2013 , (OECD, Test No. 209:2010, IDT) 2014 32290 2013 II, 1.0 92 . 1.2 2009 . , - . , , -, 1 - , , - ( ) 2 3 , (- 58- 28 2013 .) : ( 3166) 004 97 ( 3166) 004 97 AZAMBYKZKGMDRUUZUA - 4 28 2013 . 782- 32290-2013 - 1 2

2、014 .5 OECD Test No/ 209 Activated S d e, Res i atio I i itio Test (Ca o a d A o i O idatio ) ( - ). (e ). (IDT).6 - , - . ( ) - . , , 2014 -, 32290 2013 III () . - , . , , . - , - , . TG 301 TG 310. , 1. -, EC50 . , , , , , - . - . , , , (ATU) (CAS 109-57-9), , 1. , EC50 ( ) () . , - , . Nitrosomon

3、as, -. - . - , . . . 32290 2013 IV1 32290 2013 2014 08 011 ( ) ( () ) . 2 .2.1 NOEC ( ): , . , NOEC, - (p 100 /.6.4 6.4.1 ECx ECx 95 %- ( , , ). ECx - x, %, , . EC50 ECx, .6.4.2 NOEC NOEC. - ( ). - () p 0,05. 32290 2013 126.5 6.5.1 : - , , CAS, ; - - (log Kow, , , (H) , -, ); 6.5.2 : - , , , .6.5.3

4、:- , pH .6.5.4 : - ( O2 / ); - , EC50; - EC50 95%- , EC20, EC80; NOEC , EC50 ; - , ; - - ; - , ; - , . 32290 2013 131 Brown, D., Hitz, H.R., Sch fer, L. (1981), The assessment of the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria, Experience with a screening test, Chemospher

5、e 10 (3): p.245 261. 2 King, E. F. and Painter H. A. (1986), Inhibition of respiration of activated sludge; variability and reproducibility of results, Toxicity Assessment 1(1): 27 39. 3 OECD (1984), Activated sludge, Respiration inhibition test, Test Guideline No. 209, Guidelines for the testing of

6、 chemicals, OECD, Paris. 4 ISO (2007), ISO 8192 Water Quality- Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation, International Organization for Standardization. 5 Bealing, D. J. (2003), Document ISO/TC147/WGI/N.183, International Organization for

7、Standardization. 6 Painter, H A, Jones K (1963), The use of the wide-bore dropping-mercury electrode for the determination of the rates of oxygen uptake and oxidation of ammonia by micro-organisms, Journal of Applied Bacteriology 26 (3) 471 483. 7 Painter, H. A. (1986), Testing the toxicity of chemi

8、cals by the inhibition of respiration of activated sludge. Toxicity Assessment 1:515 524. 8 Robra, B. (1976), Wasser/Abwasser 117, 80. 9 Fiebig S. and Noack, U. (2004), The use of copper(II)sulphate pentahydrate as reference substance in the activated sludge respiration inhibition test - acc. to the

9、 OECD guideline 209. Fresenius Environmental Bulletin 13 No. 12b: 1556 1557. 10 ISO (1995), ISO 10634 Water Quality Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in aqueous medium, International Organizati

10、on for Standardization. 11 OECD (2006), Current approaches in the statistical analysis of ecotoxicity data: a guidance to application, Series on testing and assessment No. 54, ENV/JM/MONO(2006)18, OECD, Paris. 32290 2013 14 658.382.3:006.354 71.040.50 58 : , , , -, , . 32290 2013 15 01.09.2014. 60x841/8. . . . 2,32. 37 . . 3234. , 123995 , ., 4.www.gostinfo.ru infogostinfo.ru 32290 2013 16

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