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2、cts Determination of the lipase activity of pregastric lipase preparation ( -. ). SC 5 - ISO/TC 34 (ISO). (en). , , . (IDT)6 - , - . () - . , , 2015 -, II ISO 130822014 ( 3.1.1.3) , - , . (- ) , , . - , Romano, Provolone Asiago, , - , 294 IDF 6. Feta, Feta. FCCIV 7, FCCIV , - . FCCIV .III ISO 130822
3、014 Milk and milk products. Determination of the lipase activity of pregastric lipase preparation 201601011 . , . , - . , , .2 .2.1 , ILU (international lipase unit, ILU): -, 1,25 / .1 (ILU) 1 , 1 3.2 . , (4 %), .3 - , , (-). ILU 1 3 ILU 1 , . , - () .4 , , - , -. . , , , , .1 ISO 130822014 4.1 (),
4、Merck, 1.01958.01001), - .4.2 , Sigma C86541), .4.3 , BDH Prod. 298631), .4.4 . , ( ), Merck, 7174.10001), .4.5 , Carbosorb1), BDH, 3311041), - .4.6 , , c (NaOH) = 0,025 /3, -, . (5.1) 25,00 3 - 1 /3 1000 3. . NaOH 0,025 /3 , - , CO2 (4.5) 1 . . - , . NaOH 0,010 /3 0,025 /3. - NaOH 0,010 /3 . NaOH 0
5、,010 /3, ( ). (1).4.7 , 10 %. 100 310,0 95 3 . 12 . , - . .4.8 . - . . - . , , - , - . . .5 , , . , - .5.1 , 1 10 3, 0,5 % .5.2 , 3, A.5.3 , , - (42,0 c177 0,5) C.1) , . - ISO.2 ISO 1308220145.4 Warren1), Ultraturax1) .5.5 , pH-, :) , - ;b) ;c) , . Metrohm 718 Stat Titrino1). - , . .5.6 Stomacher St
6、omacher, , BA 6041, Seward1), .6 . . , . 2. 5 C 2 . - , 18 C, .7 7.1 600 (4.2) 95 -. 0,5 3- (4.3) 1,0 3 (4.1). 60 . - , . 4 .7.2 7.2.1 100 3(5.2) - 100 3 (4 c177 1) ILU/3. . , , 5 ILU .7.2.2 . - . - 100 3 (4 c177 1) ILU/3. 90 3 . pH , , - (8,50 c177 0,10) 0,1 /3, , . 20 100 3(5.2). . . - , 2 . - d ( 3 3 3).1) , . - ISO.3 ISO 130822014 , pH. - pH . -, pH . .7.3 . 40 3 Stomacher (5.6) (15 c177 1) . pH - (8,50 c177 0,10) . NaOH 0,1 /3. - Stomacher (5.6), 230 / 60 . pH 8,5 . , 2 . Stomacher - 60 . , , . d (8.1) : - ( ),