KS H ISO 13366-1-2006 Milk-Enumeration of somatic cells-Part 1:Microscopic method 《牛奶 体细胞的菌落计数 第1部分 显微法(参考方法)》.pdf

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1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 11 20 http:/www.kats.go.krKS H ISO 133661 1: KS H ISO 13366 1: 2006 (2011 ) H ISO 13366 1: 2006 : ( ) ( ) ( ) ( ) : (http:/www.standard.go.kr) : :2001 12 22 :2006 11 20 :2011 12 29 2011-0652 : : ( 02-509-7273) (http:/www.kats.go.kr). 10 5 , . ICS 67.10

2、0.10 KS 1: H ISO 13366 1: 2006(2011 ) MilkEnumeration of somatic cells Part 1:Microscopic method 1997 1 ISO 13366 1 Milk Enumeration of somatic cells Part 1: Microscopic method . ISO 13366 1 ISO/TC 34, (IDF) (AOAC International) , . KS H ISO 13366 . 1: 2: 3: KS H ISO 13366 A . ISO , . , . . 1. . . 2

3、 . 2.1 , . 3. . . mL (working factor) . 4. (tetrachloroethane) , (ethidium bromide) . . , , . 4.1 4.1.1 H ISO 13366 1: 2006 2 95 % (V/V) (glacial) 54.0 mL 40.0 mL 0.6 g 6.0 mL , (KS H ISO 13366 3 ). 4.1.2 65 (5.1) . . 4 . (5.3) . . 5. . 5.1 (655) . 5.2 (355) . 5.3 1012 m . 5.4 5001 000 . 5.5 0.01 m

4、L 2 % . 5.6 20 mm5 mm , 20 mm5 mm 5.7 (4010) . 5.8 . 6. 6.1 . , KS H ISO 707( 1) . 6.2 , . 6.3 26 . 6.4 6 (boric acid) , 100 mL 0.6 g . 26 , 24 . 7. 35 (5.2) . , 20 . 8. . (5.6) (dust-free paper), . 8.1 (5.5) (7. ) 0.01 mL , . 20 mm5 mm , . . . H ISO 13366 1: 2006 3 10 . , . . 8.2 (5.4) ( 400 ). , (

5、視域 ) . , . , . 9. 9.1 5 mm , (視域 ) . 0.01 mL wf . wf=bd 10020 d: (mm) b: 9.2 wf mL . 10. 10.1 KS H ISO 13366 3 B . 10.2 . (coefficient of variation) . 80 % neutrophils mL 400 000 600 000 5 % . 400 . (Poisson) . M = V = sd 2 M: V: sd: (CV) . CV =Ms 100d% CV =d100s% CV = M100% , M ( ) . H ISO 13366 1:

6、 2006 4 11. . ( ) . . H ISO 13366 1: 2006 5 A() 1 KS H ISO 707: 2006 2 KS H ISO 13366 2: 2006 2: 3 KS H ISO 13366 3: 2006 3: 1: 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS H ISO 13366 1: 2006 MilkEnumeration o f somatic cellsPart 1:Microscopic methodICS 67.100.10

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