1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 15088(zebrafish) (Danio rerio) KS I ISO 15088 :2009 2009 12 18 http:/www.kats.go.krKS I ISO 15088:2009 : ( ) ( ) ( ) : (ISO/TC147) () ( ) () () : (http:/www.standard.go.kr) ： ：2009 12 18 2009-0831 ： ：(ISO/TC147) ( 02-509-7266) (http:/www.kats.go.kr
2、). 10 5 , . KS I ISO 15088:2009 (zebrafish) (Danio rerio) Water qualityDetermination of the acute toxicity of waste water to zebrafish eggs(Danio rerio) 2007 1 ISO 15088, Water qualityDetermination of the acute toxicity of waste water to zebrafish eggs(Danio rerio) , . . . . . , . 1 48 , . . . , (：K
3、S I ISO 73461 KS I ISO 73462). , . 2 . . ( ) . KS I ISO 566716, 16： KS I ISO 15088:2009 2 KS I ISO 5814, KS I ISO 73461, 1： KS I ISO 73462, 2： 3 . 3.1 3.2 D 1 000 mL 250 mL (25 % ) D4 . KS I ISO 566716 3.3 EC 50 50 % LC 50 50 % . 3.4 (fish egg) ( ) , : “ ”; 4 128 “ ”; (embryo) “ (embryo)” . 3.5 LID
4、, , D(3.2) 3.6 LID egg 90 % 11., 12. 2 KS I ISO 15088:2009 3 3.7 4 . , . (8.2.2) (7.10, ) (microplate) , . 5 , D . 48 , (LID) . 26 72 96 . , 3.7 mg/L , 3,4- 10 (8.3) . , EC 50 . 6 . 6.1 , ( 10 S/cm) 6.2 , c(HCl)0.1 mol/L 6.3 , c(NaOH)0.1 mol/L 6.4 3,4-, , (C 6 H 5 Cl 2 N)100 mg/L (6.5) 500 mL 0.05 g
5、 24 . pH 7.0 . , 6 . 8.3 3,4- 3.7 mg/L . 6.5 KS I ISO 73461 KS I ISO 73462 . KS I ISO 15088:2009 4 294.0 mg/L (2), CaCl 2 2H 2 O 123.3 mg/L (7), MgSO 4 7H 2 O 63.0 mg/L , NaHCO 3 5.5 mg/L , KCl (6.4) , (261) 100 % . 7 7.1 / , 30 7.2 , 2.5 mL 5 mL , (24 wells) 7.3 , . 7.4 (261) , . 7.5 pH 7.6 , KS I
6、ISO 5814 7.7 7.8 7.9 , 7.10 7.11 7.11.1 7.11.2 7.11.3 7.11.4 7.11.5 , 150 mL 7.12 7.13 8 KS I ISO 15088:2009 5 8.1 8.1.1 , 6 24 . 6 ( ) . 8.1.2 (, 1 L). 8.1.3 (6.5) . (261) 80 % . ( ) . 8.1.4 . (Artemia spec.nauplia), (Daphnia) . . . . . 8.1.5 . , 16/8(/) 12/12 . 8.2 8.2.1 30 . 2 ：1 . (zebrafish) .
7、, , . , (7.8) . 4 - 128- KS I ISO 15088:2009 6 (7.7) . (4- 128- ) . , 30 . , . 50 % . (8.2.2). 8.2.2 , . : . (animalic pole) ( ) , 26 15 . , 4 , 8, 16 32 ( , 1 ). 4- . 4 - 128- . 1 . , (, ) . . a) 2- b) 4- c) 8- 1 8.3 KS I ISO 566716 . pH . , (6.2) (6.3) pH 7.00.2 . . KS I ISO 15088:2009 7 . pH ( ,
8、) pH , . 1 1 . 1 , D X D D % 0 1 100.00 1 2 50.00 2 3 33.00 3 4 25.00 5 6 16.67 7 8 12.50 11 12 8.33 15 16 6.25 23 24 4.17 31 32 3.13 O 2 4 mg/L O 2 ( 50 % ) . , O 2 . , 40 ( 100 mL ) . . (, 24 2 mL) . 10 . 24 , 2 . , 4 . , 10 ( A ). , 10 3,4- 3.7 mg/L . , (261) 48 . , / . 9 9.1 KS I ISO 15088:2009
9、8 48 , , . 9.2 . . 9.3 . 2 . “ ” . 9.4 2 “a” . . 24 48 a b 2 24 48 . . . 48 , . , ( 48 , 24 ,“ ” . 2 ) 10 a b a bKS I ISO 15088:2009 9 . 90 % 48 , ( 3.7 mg/L 10 % , 8.3 ) , (3,4- 3.7 mg/L) 10 (8.3) . 11 LID egg 48(9.2) . 9.4 , . 90 % , D(LID) . . , . . . (0.1 % ). , . , . 12 LID . LID egg 2 , EC 50
10、. ISO/TS 20281 . 13 . . a) (KS I ISO 15088) b) c) d) pH (mg/L ), pH e) 12. f) 14 KS I ISO 15088:2009 10 2000 . 3,4- (3,4- 3.7 mg/L) 2 . . http:/www.gdch.de/strukturen/fg/wasser/publikat/vali/vdok_t6.pdf 1) . 2 3,4- EC 50 3,4- 3.7 mg/L l n o % x s RCV R % s rCV r % A 15 43 8.5 3.31 mg/L 0.455 mg/L 13
11、.8 0.289 mg/L 8.7 B 16 46 0.0 68.0 % 24.0 % 35.0 16.9 % 25.0 l ： n ： o ： x ： s R： CV R： s r： CV r： ： A 3,4-DCA EC 50 (mg/L) B 3,4-DCA 3.7 mg/L (%) 1) . . KS I ISO 15088:2009 11 A () 1 (3.7 mg/L, 3,4-DCA) 2 3 4 A.1 1 2 3 4KS I ISO 15088:2009 12 1 ISO/TS 20281, Water qualityGuidance on statistical int
12、erpretation of ecotoxicity data 2 DETRICH, H.W., WESTERFIELD, M., ZON, L.I. The zebrafish biology. Methods in Cell Biology, 59, Academic Press, San Diego, London, Boston, New York, Sydney, Tokyo, Toronto, 1999, 391 pp. 3 FRICCIUS, T., SCHULTE, C., ENSENBACH, U., SEEL, P. and NAGEL, R. Der Embryotest
13、 mit dem Zebrabrbling-eine neue Mglichkeit zur Prfung und Bewertung der Toxizitt von Abwasserproben. Vom Wasser, 84, 1995, pp. 407-418 4 FUMANN, G. and NAGEL, R. LaborvergleichsversucheEmbryotest mit dem Zebrabrbling Brachydanio rerio. UBA-FB 98-009, 1997 5 HISAOKA, K.K. and FIRLIT, C.F. Further stu
14、dies on the embryonic development of the zebrafish, Brachydanio rerio (Hamilton Buchanan). J. Morphol., 107, 1960, pp. 205-225 6 HISAOKA, K.K. and BATTLE, H.J. The normal developmental stages of the zebrafish, Brachydanio rerio (Hamilton Buchanan). J. Morph., 102, 1958, pp. 311-328 7 KIMMEL, C.B., B
15、ALLARD, W.W., KIMMEL, S.R., ULLMAN, B. and SCHILLING, T.F. Stages of Embryonic Development of the Zebrafish. Developmental Dynamics, 203, No. 3, 1995, pp. 253-310 8 NAGEL, R. and ISBERNER, K. Testing of chemicals with fish a critical evaluation of tests with special regard to zebrafish. In: Fish Eco
16、toxicology (eds. BRAUNBECK, T., HINTON, D.E. and STREIT, B.), Birkhuser Verlag, Basel, Switzerland, 1998, pp. 338-352 9 SCHIRONE, R.C. and GROSS, L. Effect of temperature on early embryological development of the zebrafish, Brachydanio rerio. J. exp. Zool., 169, 1963, pp. 43-52 10 SCHULTE, C. and NA
17、GEL, R. Testing Acute Toxicity in the Embryo of Zebrafish, Brachydanio rerio, as an Alternative to the Acute Fish Test: Preliminary results. ATLA, 22, 1994, pp. 12-19 11 WESTERFIELD, M. The zebrafish book. Guide for the laboratory use of zebrafish (Danio rerio). 3rd edn., Univ. of Oregon Press, Euge
18、ne, 1995 12 OECD Guideline 203, FishAcute toxicity test 13 OECD Guideline 204, FishProlonged toxicity test: 14-day study 14 OECD Guideline 210, FishEarly-life stage toxicity test (zebrafish) (Danio rerio) 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KS I ISO 15088 :2009 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water quality Determination of the acute toxicity of waste water to zebrafish eggs(Danio rerio) ICS 13.060.70 Korean Agency for Technology and Standards http:/www.kats.go.kr
