江西省万载县高中生物专题1基因工程1.1DNA重组技术的基本工具2教案新人教版选修3.doc

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1 -1.1 DNA 重组技术的基本工具 2教学目标 基因工程载体需要具备的条件重点 基因工程载体需要具备的条件难点 基因工程载体需要具备的条件教学过程 时间 设计意图 个人备课一、 检查预习检查世纪金榜自主预习【1】完成情况。二、导入新课复习导入三、推进新课1、载体需要的条件:有一至多个限制酶切点对受体细胞无害在宿主细胞中能保存下来并大量复制有特殊的标记基因,便于筛选2、常用运载体:质粒噬菌体的衍生物或某些动植物病毒3、基因工程中常用的载体质粒(1)特点:质粒是一种裸露的、小型、双链、环状 DNA 分子。(2)本质:质粒是独立于细菌拟核 DNA 之外的小型 DNA 分子,不是细胞器。4.载体的作用:(1)作为运输工具,将目的基因导入受体细胞。(2)质粒携带目的基因在受体细胞内大量复制。5、重组 DNA 分子的模拟操作四、课堂训练【例】在基因工程中用来修饰改造生物基因的工具是 ( )3130检查预习情况,为讲解新课铺垫。导入新课,口述学习目标讲授分析,掌握基因工程载体需要具备的条件- 2 -A.限制酶和 DNA 连接酶 B.限制酶和水解酶C.限制酶和载体 D.DNA 连接酶和载体【解析】选 A五、课堂小结六、 作业和预习1、完成世纪金榜课时检测区 P42、预习基因工程的基本操作程序641当堂训练,检测知识运用总结本课学习内容,学生记忆相关知识点。布置下节课的预习任务教学反思

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