1、C 52 GBZ GBZ/T 160.81 2004 Methods for determination of biological compounds in the air of workplace 2004 5 21 2004 12 1 GBZ/T 160.81 2004 GBZ 1 GBZ 2 2004 12 1 GBZ/T 160.81 2004 1 2 GBZ 159 3 3.1 Ab1 Ab2 Ab3 3.2 3.2.1 3.2.2 40mm 3.2.3 25mm 3.2.4 0 10L/min 3.2.5 50ml 3.2.6 3.2.7 3.2.8 3.2.9 3.2.10 2
2、5ml 3.2.11 10ml 3.2.12 3.3 3.3.1 pH 9.6 0.2 1.51g 2.93g 1000ml 2 3.3.2 29.22g 1.86g Tris 1.0g (BSA) 1000ml (6mol/L) pH 8.0 0.5ml 20 1 3.3.3 - pH 5.0 0.2 7.30g 23.87g (Na 2 HPO 4 12H 2 O) 1000ml 1 5.4 BSA 2.0g BSA 100ml 3.3.5 29.22g 0.93g Tris 4.96g (Na 2 S 2 SO 3 5H 2 O) 0.147g (CaCl 2 2H 2 O) 800ml
3、 1.0g BSA (6mol/L) pH 8.0 1000ml 1.0ml 20 1 3.3.6 OPD pH 5.0 8.0mg OPD 50ml 15ml - 5 l (30 ) 3.3.7 10ml 10 l 3.3.8 10ml 10 l 3.3.9 10ml 10 l 3.3.10 1mol/L 55.5ml ( 20 1.84g/ml) 900ml 3.4 GBZ 159 3.4.1 5L/min 15min 3.4.2 1L/min 2 8h 3.4.3 1L/min 2 8h 2 3.5 3.5.1 3.5.2 25ml 20min 3.5.3 3.5.3.1 8 0 0.3
4、 0.6 1.2 2.4 3.64.8 6.0ng/ml 3.5.3.2 100 l 4 3 250 l 200 l BSA 1h 2 3.5.3.3 50 l 50 l 37 90min3 250 l 100 l 37 90min 3 250 l - 3 100 l OPD 37 150 l 492nm 620nm 3.5.3.4 ng/ml 3.5.4 (ng/ml) 3.6 3.6.1 1 2 93 P V o = V 1 273 + t 101.3 Vo L V L t P kPa 3.6.2 2 25 c C = 2 Vo C g/m 3 c ng/ml 25 ml Vo L 3.6.3 GBZ 159 3.7 3.7.1 0.05ng/ml 0.017g/m 3 75L 0.05 6ng/ml 2 8 3.7.2 95 90 3.7.3
