1、BRITISH STANDARD BS EN ISO 14902:2001 Animal feeding stuffs Determination of trypsin inhibitor activity of soya products The European Standard EN ISO 14902:2001 has the status of a British Standard ICS 65.120 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBS EN ISO 14902:2001
2、This British Standard, having been prepared under the direction of the Consumer Products and Services Sector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 15 November 2001 BSI 15 November 2001 ISBN 0 580 38674 0 National foreword T
3、his British Standard is the official English language version of EN ISO 14902:2001. It is identical with ISO 14902:2001. The UK participation in its preparation was entrusted to Technical Committee AW/10, Animal feeding stuffs, which has the responsibility to: A list of organizations represented on
4、this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”
5、, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself conf
6、er immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulga
7、te them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN ISO title page, the EN ISO foreword page, the ISO title page, pages ii to iv, pages 1 to 11 and a back cover. The BSI copyright date displayed in this document indicates when the document was las
8、t issued. Amendments issued since publication Amd. No. Date CommentsEUROPEANSTANDARD NORMEEUROPENNE EUROPISCHENORM ENISO14902 October2001 ICS65.120 Englishversion AnimalfeedingstuffsDeterminationoftrypsininhibitoractivity ofsoyaproducts(ISO14902:2001) AlimentsdesanimauxDosagedelactivitdesinhibiteurs
9、 trypsiquesdesproduitsdesoja(ISO14902:2001) FuttermittelBestimmungderTrypsinInhibitorenAktivitt inSojaerzeugnissen(ISO14902:2001) ThisEuropeanStandardwasapprovedbyCENon3October2001. CENmembersareboundtocomplywiththeCEN/CENELECInternalRegulationswhichstipulatetheconditionsforgivingthisEurope an Stand
10、ardthestatusofanationalstandardwithoutanyalteration.Uptodatelistsandbibliographicalreferencesconcernings uchnational standardsmaybeobtainedonapplicationtotheManagementCentreortoanyCENmember. ThisEuropeanStandardexistsinthreeofficialversions(English,French,German).Aversioninanyotherlanguagemadebytra
11、nslation undertheresponsibilityofaCENmemberintoitsownlanguageandnotifiedtotheManagementCentrehasthesamestatusasthe official versions. CENmembersarethenationalstandardsbodiesofAustria,Belgium,CzechRepublic,Denmark,Finland,France,Germany,Greece, Iceland,Ireland,Italy,Luxembourg,Netherlands,Norway,Port
12、ugal,Spain,Sweden,SwitzerlandandUnitedKingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPISCHESKOMITEEFRNORMUNG ManagementCentre:ruedeStassart,36B1050Brussels 2001CEN Allrightsofexploitationinanyformandbyanymeansreserved worldwideforCENnationalMembers. Ref.No.ENISO14902:20
13、01EForeword ThetextoftheInternationalStandardISO14902:2001hasbeenpreparedbyTechnical CommitteeISO/TC34“Agriculturalfoodproducts“incollaborationwithTechnicalCommittee CEN/TC327“AnimalfeedingstuffsMethodsofsamplingandanalysis“,thesecretariatof whichisheldbyNEN. ThisEuropeanStandardshallbegiventhestatu
14、sofanationalstandard,eitherbypublication ofanidenticaltextorbyendorsement,atthelatestbyApril2002,andconflictingnational standardsshallbewithdrawnatthelatestbyApril2002. AccordingtotheCEN/CENELECInternalRegulations,thenationalstandardsorganizationsof thefollowingcountriesareboundtoimplementthisEurope
15、anStandard:Austria,Belgium, CzechRepublic,Denmark,Finland,France,Germany,Greece,Iceland,Ireland,Italy, Luxembourg,Netherlands,Norway,Portugal,Spain,Sweden,SwitzerlandandtheUnited Kingdom. NOTEFROMCMC TheforewordissusceptibletobeamendedonreceptionoftheGermanlanguage version.Theconfirmedoramendedforew
16、ord,andwhenappropriate,thenormativeannexZAforthe referencestointernationalpublicationswiththeirrelevantEuropeanpublicationswillbecirculatedwith theGermanversion. Endorsementnotice ThetextoftheInternationalStandardISO14902:2001wasapprovedbyCENasaEuropean Standardwithoutanymodification. ENISO14902:200
17、1 Reference number ISO 14902:2001(E) INTERNATIONAL STANDARD ISO 14902 First edition 2001-10-15 Animal feeding stuffs Determination of trypsin inhibitor activity of soya products Aliments des animaux Dosage de lactivit des inhibiteurs trypsiques des produits de soja ENISO14902:2001 ii ENISO14902:2001
18、 ISO 20941:(1002)Eiii Contents Page Foreword.iv 1 Scope 2 2 Normative reference 2 3 Term and definition .2 4 Principle2 5 Reagents and materials 2 6 Apparatus .3 7 Sampling.4 8 Preparation of test sample4 9 Procedure .4 9.1 Number of determinations 4 9.2 Sample extraction4 9.3 Dilution of sample ext
19、ract.5 9.4 Measurement of trypsin activity of working solution 5 9.5 Measurement of trypsin inhibitor activity .5 10 Calculation6 10.1 Inhibition percentage of sample extract solutions.6 10.2 Trypsin inhibitor activity.7 11 Precision.7 11.1 Interlaboratory tests 7 11.2 Repeatability.7 11.3 Reproduci
20、bility.8 12 Test report 8 Annex A (normative) Dilution scheme for sample extract9 Annex B (informative) Results of interlaboratory test.11 Bibliography12 ENISO14902:2001 ISO 20941:(1002)E iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national stand
21、ards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International orga
22、nizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given
23、in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility
24、that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO 14902 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs. Annex A forms a normative part of this International Standard. Annex B is for information only. ENISO14902:2001
