1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 10229 ( )KS I ISO 10229:2008 2008 11 24 http:/www.kats.go.krKS I ISO 10229:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 1998 3 26 ： 2008 11 24 2008-0794 ： ： ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 10229:2008 ( ) Water quality D
2、eter mination of the prolonged toxicity of substances to freshwater fish Method for evoluting the effects of substances on the growth rate of rainbow traoutOncorhynchus mykiss Walbaum(Teleostei, Salmonidae) 1994 1 ISO 10229, Water quality Deter mination of the prolonged toxicity of substances to fre
3、shwater fish Method for evoluting the effects of substances on the growth rate of rainbow traoutOncorhynchus mykiss Walbaum(Teleostei, Salmonidae) . 1 Oncorhynchus mykiss Walbaum(Teleostei, Salmonidae)- . 1 , . (8.2 ). 14 28 ( B 1 ). 2 , , . . . , , , , . KS I ISO 10229:2008 2 2 . . ( ) . KS I ISO 7
4、346 2, ( ) KS I ISO 7346 3, ( ) 3 14 28 ( ) . . a) KS I ISO 7346 2 KS I ISO 7346 3 (KS I ISO 7346 .) , 96 50 % . b) 96 LC50 (8.1 ). c) 16 (lowest observed effect concentration： LOEC) (no observed effect concentration： NOEC) . “28 LOEC” . IC10(10 % ) . ( 20 % ) LOEC NOEC . ( 20 %) 80 % 120 % , LOEC N
5、OEC . ( 20 % ) LOEC NOEC . LOEC xmg/L NOEC ymg/L (, y ). . (freeze-branding) (tagging ) . KS I ISO 10229:2008 3 4 Oncorhynchus mykiss Walbaum(Teleostei, salmonidae) . , 2 . , , , . (8. ) , 1 % , 4 % (8.2 ). 1 . 5 2 . 14 , . 3 g 5 g (8.2 ). 10 % . . 2 . . 2 ( B 2). 5 . . 5.1 . pH 6.7 8.5 pH 0.2 . pH
6、1 mg/L 100 mg/L CaCO3 20 mg/L 300 mg/L CaCO3 , . . 294.0 mg/L CaCl22H2O 123.3 m g/L MgSO47H2O 63.0 mg/L NaHCO35.5 mg/L KCl 5.2 , . , , (1. 1 ) , KS I ISO 10229:2008 4 . . . , , . , 0.1 mL/L( 0.01 mL/L ) 2 . , 2 . . 5.3 . 10 L 100 mL . . 6 6.1 , . ( .)(6.2 ) . 6.2 45 L , . (5.1) . ( .) . (5.1) . 6.3
7、12.5 17.5 1 . 6.4 10 % . KS I ISO 10229:2008 5 (air saturation value： ASV) 70 % . 6.5 . ( 22 SWG 316) . 1 . . 1 2 6 . . . 2 . 1 7 , . . 12 16 . 8 8.1 KS I ISO 7346 2 KS I ISO 7346 3 96 LC50 . . , . KS I ISO 10229:2008 6 . KS M ISO 7346 2 KS M ISO 7346 3 8.( ) . 8.2 5 . 8.1 96 LC50 10 % 32 % . 5 . 1
8、96 LC50 , LC50 10 % . LC50 96 96 32 % . 10 (3.162) . 2 . 45 L 6 (6.36.5) 1 2 40 L (5.3). (5.1). . (6.3 ). , , . . 4 . 3 1 2 2 0 1 2 . 07 3 814 4 15 21 5 22 28 6 200 L/day (6.4). 5 g . ASV 70 % , (6.4 ). . . KS I ISO 10229:2008 7 2 . a) . b) . 4. . . c) 24 (4.). 100 mg/L ( ) . 100 mg mm ( , ). B 4 .
9、. 3 . . 16 . . . . , 4 . 14 24 . , , (3. ) . 14 24 , , . . . ( ). 1 . 2 10 % 96 LC 50 . 1 , pH, . . d) . 14 , 28 . . 9 , 3,4- (ring) KS I ISO 10229:2008 8 29 % 31 % . 11 6 ( B 3 ). 10 10.1 . a) (ASV) 70 % . b) 12.5 17.5 2 . c) 20 % (3. ). d) 10 % . 10.2 0 , 14 , 28 . a) b) c) (r) . 100loglog12ttmmrt
10、1et2e mt1 ： t1 (g) mt2 ： t2 (g) mt1 mt2 ： . r . t1 0 t2 14 t1 14 t2 28 t1 0 t2 28 10.3 LOEC NOEC LOEC NOEC , , . . Dunnett William . , LOEC IC10 . KS I ISO 7346 1 7.2(LC50 ) . EC10( 10 % ) IC10 . KS I ISO 10229:2008 9 11 . a) (KS I ISO 10229) b) c) , d) , , , e) 1) 2) f) , , 8.2 ( ) g) , , r , h) LO
11、EC NOEC IC10 (10.3 ) ( 2 .) i) j) A . KS I ISO 10229:2008 10 99 % 95 % 3,4- (g/L) 2 3,4- 14 28 0 14 15 28 0 28 KS I ISO 10229:2008 11 A ( ) A . B . pH 1 3 A . 1 B . KS I ISO 10229:2008 12 A (0 ) ( /L) KS I ISO 10229:2008 13 B (0 ) mg/L mL/hrpH KS I ISO 10229:2008 14 B ( ) 1 CROSSLAND, N.O. A method
12、to evaluate effects of toxic che micals on fish growth, Che mosphere 14,(11/12)(1985), pp. 1855 1870 2 BARNABE, G. Aquaculture, Volume 2. Technique et Docu mentation Lavoisier, Paris(1986) 3 ASHLEY, S. and Mallett, M.J. EEC ring test of a method for determining the effects of che micals on the growt
13、h rate of fish. Report to the Commission of the European Communities No. EEC 2600-M(1990), Water Research Centre, U.K 4 A GLER, K. F. Capture, sampling and examination of fishes, pp. 48 83, in Methods for assessment of fish production in fresh waters, edited by T. Bagenal. IBP Handbook No. 3, 3rd ed
14、ition(1978). Blackwell Scientific Publications Oxford 5 DUNNETT, C. W. New tables for multiple co mparisons with a control. Bio metrics 20(1964), pp. 482 491 6 WILLIA MS, D.A. A test for differences between treat mentmeans when several dose levels are compared with a zero dose control. Biometries 27
15、(1971), pp. 103 117 ( ) 135513 7017 (02)60094114 (02)600948878 http:/ KS I ISO 10229:2008 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water qualityDetermination of the prolonged toxicity of substances to freshwater fishMethod for evoluting the effects of substances on the growth rate of rainbow troutOncorhynchus mykiss Walbaum(Teleostei, Salmonidae) ICS 13.060.01 Korean Agency for Technology and Standards http:/www.kats.go.kr
