1、The Impact of Climatic Factors on Fecal Coliforms/Fecal Streptococci Ratio Variability,Kellie E. Jones Longwood College Department of Natural SciencesCoauthor: Genii A. Grimsley Faculty Advisor: Dr. David Buckalew,Fecal Coliforms Vs. Fecal Streptococci,FC/FS Source of Pollution,Gerba, Charles P., et
2、. al. Environmental Microbiology. New York: Academic Press,1999.,Research Hypothesis,FC/FS ratioDuring moderate climatic conditions variability decreases Enhances the application of the ratio as a bacterial source indicator,Method,Collection of water samples Presumptive test for fecal streptococci P
3、late count Confirmatory tests for fecal streptococci,Presumptive Test,Membrane Filtration KF Streptococcal Broth Incubate at 37 +/- 0.5 C for 48 +/- 2 hrs Perform Plate Count Calculate FC/FS ratios,Confirmatory Test,Bile Esculin Agar Incubate at 44.5 C +/- 0.5 C for 18 hrs Observe Growth,FC/FS Ratio
4、s for September - April,T test Results for November March vs. September and October,Ang 17.0 1.510 0.25p0.10 App 1.0 1.281 0.50p0.25 App 2.0 0.529 1.00p 0.50 Say 5.0 1.560 0.25p0.10 Say 8.0 1.237 0.50p0.25 Vau 14.0 1.369 0.25p0.10,T test Results for November March vs. September, October, and April (
5、1),Ang 17.0 1.2607 0.50p0.25 App 1.0 0.9843 0.50p0.25 App 2.0 0.8104 0.50p0.25 Say 5.0 1.4487 0.25p0.10 Say 8.0 1.3701 0.25p0.10 Vau 14.0 1.1762 0.25p0.10,T test Results for November March vs. September, October, and April (2),Ang 17.0 1.77 0.25p0.10 App 1.0 0.977 0.50p0.25 App 2.0 0.8104 0.50p0.25
6、Say 5.0 1.8448 0.25p0.10 Say 8.0 1.3701 0.25p0.10 Vau 14.0 1.424 0.25p0.10,Discussion,Already see a shift in p values with the addition of Aprils dataWith more research the critical value could possibly be reached and there may actually be less variability in moderate monthsMonthly variation in temperature and precipitation counteract the reliability of the FC/FS ratio,Conclusion,We feel that it is important to continue research on the FC/FS ratios because they are the most inexpensive source indicators of non-point bacterial contamination in waterways.,
