1、raising standards worldwide NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW BSI Standards Publication BS ISO 9345-1:2012 Microscopes Imaging distances related to mechanical reference planes Part 1: Tube length 160 mmBS ISO 9345-1:2012 BRITISH STANDARD National foreword This Br
2、itish Standard is the UK implementation of ISO 9345-1:2012. It supersedes BS 7012-3:1997 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee CPW/172/5, Optics and Photonics - Microscopes. A list of organizations represented on this committee can be obtain
3、ed on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2012. Published by BSI Standards Limited 2012 ISBN 978 0 580 77421 8 ICS 37.020 Compliance wit
4、h a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2012. Amendments issued since publication Date T e x t a f f e c t e dBS ISO 9345-1:2012 ISO 2012 Microscopes Imaging
5、distances related to mechanical reference planes Part 1: Tube length 60 Microscopes Tirages mcaniques en fonction des plans mcaniques de rfrence Partie 1: Longueur de tubes de 160 mm INTERNATIONAL STANDARD ISO 9345-1 Second edition 2012-06-15 Reference number ISO 9345-1:2012(E)BS ISO 9345-1:2012ISO
6、9345-1:2012(E) ii ISO 2012 All rights reserved COPYRIGHT PROTECTED DOCUMENT ISO 2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permiss
7、ion in writing from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in SwitzerlandBS ISO 9345-1:2012ISO 9345-1:20
8、12(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for whi
9、ch a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all m
10、atters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are cir
11、culated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held respon
12、sible for identifying any or all such patent rights. ISO 9345-1 was prepared by Technical Committee ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes. This second edition cancels and replaces the first edition (ISO 9345-1:1996) which has undergone a minor revision to al
13、ign definitions with ISO 10934-1 and to adjust presentation to ISO 9345-2. ISO 9345 consists of the following parts, under the general title Microscopes Imaging distances related to mechanical reference planes: Part 1: Tube length 160 mm Part 2: Infinity-corrected optical systems ISO 2012 All rights
14、 reserved iiiBS ISO 9345-1:2012BS ISO 9345-1:2012Microscopes Imaging distances related to mechanical reference planes Part 1: Tube length 160 mm 1 Scope This part of ISO 9345 specifies the imaging distances of objectives and eyepieces of microscopes with 160 mm mechanical tube length or equivalent.
15、NOTE A specific combination of eyepiece and objective is frequently used to correct aberrations. Therefore, the combination of an objective from one manufacturer and an eyepiece from another manufacturer, although conforming to this International Standard, might cause loss of image quality. 2 T erms
16、 and definitio ns For the purposes of this document, the following terms and definitions apply. 2.1 parfocalizing distance of the objective distance in air between the object plane (i.e. the uncovered surface of the object) and the locating flange of the objective, when the microscope is in its work
17、ing position Note 1 to entry: The parfocalizing distance of the objective is one of the optical interfacing dimensions. ISO 10934-1:2002, definition 2.80.2.4 2.2 objective to primary image distance distance in air between the objective locating surface (of the nosepiece) and the primary image plane
18、Note 1 to entry: The objective to primary image distance is one of the optical interfacing dimensions and commonly has a value of either 150 mm or infinity. The latter is a hypothetical value applied to microscopes designed for infinity- corrected objectives. ISO 10934-1:2002, definition 2.80.2.1 2.
19、3 parfocalizing distance of the eyepiece distance between the locating flange of the eyepiece and the plane upon which the eyepiece is focused Note 1 to entry: The plane upon which the eyepiece is focused is coincident with the plane of the final real image of the microscope when the eyepiece is mou
20、nted in the viewing tube. The parfocalizing distance of the eyepiece is one of the optical interfacing dimensions, and is commonly 10 mm. ISO 10934-1:2002, definition 2.80.2.3 2.4 mechanical tube length for objectives corrected for a finite primary image distance it is the length of the tube in its
21、simplest form (i.e. without any intermediate lenses) and is the distance in air between the objective-locating surface of the nosepiece and the eyepiece-locating surface of the viewing tube Note 1 to entry: The mechanical tube length is one of the optical interfacing dimensions of the microscope. IN
22、TERNATIONAL STANDARD ISO 9345-1:2012(E) ISO 2012 All rights reserved 1BS ISO 9345-1:2012ISO 9345-1:2012(E) Note 2 to entry: It commonly has a value of 160 mm (ISO 9345-1). Note 3 to entry: For infinity-corrected objectives, the mechanical tube length is hypothetically considered to be infinite. ISO
23、10934-1:2002, definition 2.143.1 3 Requirements 3.1 Nominal dimensions and tolerances The nominal dimensions shall be as given in Table 1 and illustrated in Figure 1. Table 1 Nominal dimensions and tolerances Feature Symbol Nominal value mm Numerical aperture Tolerance mm Parfocalizing distance of t
24、he objective l 1 45,00 0,1 0,2 0,1 to 0,25 0,06 0,25 to 0,45 0,03 0,45 0,01 Objective to primary image distance l 2 150,00 0,5 Parfocalizing distance of the eyepiece l 3 10,00 0,3 Mechanical tube length l 4 160,00 0,5 NOTE 1 The tolerance 0,2 for the parfocalizing distance of objectives with numeric
25、al aperture 0,1 does not necessarily apply to an objective with magnification lower than 4 . NOTE 2 The parfocalizing distance of 45 mm given in Table 1 and shown in Figure 1 is intended to apply to objectives when used with uncovered objects (specimens). Objectives for use with objects covered by a
26、 coverglass shall have a parfocalizing distance as follows, to allow for the virtual displacement of the object by the coverglass: where t is the thickness of the coverglass; n is the refractive index of the glass. 2 ISO 2012 All rights reservedBS ISO 9345-1:2012ISO 9345-1:2012(E) Key 1 eyepiece-loc
27、ating surface of the viewing tube 2 primary image plane 3 locating flange of the eyepiece 4 locating flange of the objective 5 objective-locating surface (of the nosepiece) 6 object plane NOTE Many microscopes have built-in prisms and lenses to change the position and/or the magnification of the ima
28、ge. In this case, the microscope shall have a construction such that, in combination with objectives conforming to this part of ISO 9345, the primary image is produced 10 mm below the eyepiece-locating surface of the viewing tube. Figure 1 Locating surfaces, reference planes and imaging distances IS
29、O 2012 All rights reserved 3BS ISO 9345-1:2012ISO 9345-1:2012(E) 3.2 Examples Figure 2 illustrates the influence of different cover glass thicknesses on the parfocalizing distance. Dimensions in millimetres Uncovered object t = 0 mm Object with cover glass t = 0,17 mm thickness n = 1,5 Object with c
30、ulture chamber t = 1,5 mm bottom thickness n = 1,5 l CG= l 1 a l CG= l 1+ 0,06 mm a l CG= l 1+ 0,5 mm a al CGis the resulting parfocalizing distance due to different cover glass thickness. Figure 2 Examples of parfocalizing distances as function of cover glass thickness 4 Marking If the magnificatio
31、n of the primary image is changed by built-in optical systems, the tube factor shall be marked on the magnification changing component (stand, tube etc.). EXAMPLE 1,25 4 ISO 2012 All rights reservedBS ISO 9345-1:2012ISO 9345-1:2012(E) Bibliography 1 ISO 8039, Microscopes Values, tolerances and symbo
32、ls for magnification 2 ISO 9345-2, Optics and optical instruments Microscopes: Imaging distances related to mechanical reference planes Part 2: Infinity-corrected optical systems 3 ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part 1: Light microscopy ISO 2012 All rights rese
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