GOST 32638-2014 OECD guidelines for the testing of chemicals In vitro mammalian cell cene mutation test《化学品试验OECD指南 体外哺乳动物细胞基因突变试验》.pdf

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1、 in vitro(OECD, Test 476:1997, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)326382014, - 1.092 . - 1.22009 . , . , , - 1 - , , - ( ); 339 , - , 42 ()3 , (- 30 2014 . 67-) : ( 3166) 00497 ( 3166) 00497 AZAMBYKZKGMDRUTJTMUZUA - 4 OECD Test 476:1997 In VitroMammalian

2、 Cell Gene Mutation Test ( in vitro). (en). (IDT)5 29 2014 . 1232- 326382014 - 1 2015 .6 - , - . () - . , , 2015 -, II 3263820141 12 13 .24 24.1 .24.1.1 24.1.2 24.1.3 .24.1.4 24.1.5 / 34.2 .34.2.1 /.34.2.2 34.2.3 34.3 .44.3.1 44.3.2 , .45.45.1 45.2 55.3 5 .7III 326382014 in vitro - , . - : L5178Y, C

3、HO, AS52 V79 ; TK6 2. - , () - (HPRT) - (XPRT). , HPRT XPRT . XPRT (. . ), - HPRT - 3, 4, 5, 6, 7.IV 326382014 in vitroOECD guidelines for the testing of chemicals. In vitro mammalian cell gene mutation test 201506011 in vitro - . . in vitro - . , in vivo. - , , . - , , , , 8. -. , , . . - . , , , ,

4、 , , 7.2 2.1 (Phenotypic expression time): , - .2.2 (Survival): ; .2.3 (Viability): .2.4 (Base pair substitution mutagens): , .2.5 ( )(Frameshift mutagens): , .2.6 (Relative total growth): - .2.7 (Relative suspension growth): - .2.8 (Forward mutation): , .2.9 (Mutant frequency): - .1 326382014 3 , (

5、) + c174 -, - (). , -, , . , . , HPRT XPRT, 6- () 8- (). in vitro - , , , -. , - . / , . - , 10, 11, 12, 13, 14. - () . - , , - . - (). . .4 4.1 4.1.1 , L5178Y, CHO, AS52, V79 TK6. , . - . . . , - , 15. - , , . , 10 . 106 . , .4.1.2 (- , , 2 ). - . , - , .4.1.3 , 37 . - .4.1.4 - . - (S9) , , Arochlor 16, 17,18, 19 c98- 20, 21. -2 326382014 110 % . . - . , , - , . (, 450, ).4.1.5 / . / . , .4.2 4.2.1 / S9 . , , . , , /-. , . .4.2.2 -, -, . - , (-) . . 4 . - . 2 10 . , 1020 % ( 10 %) ( ) . 5 /, 5 / 0,01, .

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